PMID- 10807745
OWN - NLM
STAT- MEDLINE
DCOM- 20000620
LR  - 20190831
IS  - 1079-5642 (Print)
IS  - 1079-5642 (Linking)
VI  - 20
IP  - 5
DP  - 2000 May
TI  - Induction of IG9 monocyte adhesion molecule expression in smooth muscle and 
      endothelial cells after balloon arterial injury in cholesterol-fed rabbits.
PG  - 1293-300
AB  - The expression of monocyte-specific adhesion molecules and chemokines by cell 
      types within the vessel wall plays an important role in foam cell accumulation 
      during atherosclerotic plaque development. We previously identified IG9, a novel 
      monocyte adhesion protein that is expressed on endothelial cells (ECs) overlying 
      human and rabbit advanced atherosclerotic plaques. The present study was designed 
      to determine the temporal and spatial expression of IG9 and the chemokine, 
      monocyte chemoattractant protein-1 (MCP-1), after balloon injury with (double 
      injury) or without (single injury) prior air desiccation EC injury in the femoral 
      arteries of rabbits fed a high-cholesterol diet. By immunohistochemical analyses, 
      intense reactivity with monoclonal antibodies to IG9 and MCP-1 was detected 24 
      hours after single injury in medial smooth muscle cells (SMCs) and in SMCs of 
      adventitial microvessels. However, monocyte infiltration of the tunica media was 
      minimal or not detected in these sections. IG9 and MCP-1 antibody reactivity in 
      vessel sections 28 days after single injury and 24 hours, 7 days, and 28 days 
      after double injury was localized to medial and neointimal SMCs, foam cells, and 
      luminal ECs overlying the plaques. Uninjured rabbit (cholesterol or normal diet) 
      vessel sections exhibited minimal IG9 and MCP-1 immunostaining. In vitro studies 
      using human aortic SMCs demonstrated IG9 protein induction after 24 hours of 
      treatment with platelet-derived growth factor-BB and interferon-gamma or 
      epidermal growth factor. IG9 expression was further increased by pretreatment of 
      SMCs with the proatherogenic lipid, minimally oxidized low density lipoprotein. 
      After balloon injury (24 hours), IG9 is induced in vascular SMCs before the 
      detectable accumulation of monocytes within the vessel wall. Thus, the expression 
      of IG9 by SMCs as well as by ECs may be an important factor in the accumulation 
      of foam cells in atherosclerotic plaque development after arterial injury.
FAU - Calderon, T M
AU  - Calderon TM
AD  - Department of Pathology, Albert Einstein College of Medicine, Bronx, NY, USA. 
      calderon@aecom.yu.edu
FAU - Gertz, S D
AU  - Gertz SD
FAU - Sarembock, I J
AU  - Sarembock IJ
FAU - Berliner, J A
AU  - Berliner JA
FAU - Fallon, J T
AU  - Fallon JT
FAU - Taubman, M B
AU  - Taubman MB
FAU - Berman, J W
AU  - Berman JW
LA  - eng
GR  - HL-30568/HL/NHLBI NIH HHS/United States
GR  - HL-43302/HL/NHLBI NIH HHS/United States
GR  - HL-54469/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Arterioscler Thromb Vasc Biol
JT  - Arteriosclerosis, thrombosis, and vascular biology
JID - 9505803
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cholesterol, Dietary)
RN  - 0 (Cytokines)
RN  - 0 (Growth Substances)
RN  - 0 (IG9 protein, Oryctolagus cuniculus)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (oxidized low density lipoprotein)
SB  - IM
MH  - Animals
MH  - Aorta
MH  - Carotid Artery Injuries/etiology/*metabolism
MH  - Catheterization
MH  - Cell Adhesion
MH  - Cell Adhesion Molecules/*genetics
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics
MH  - Cholesterol, Dietary/*administration & dosage
MH  - Cytokines/pharmacology
MH  - Endothelium, Vascular/*metabolism
MH  - *Gene Expression Regulation/drug effects
MH  - Growth Substances/pharmacology
MH  - Humans
MH  - Lipoproteins, LDL/pharmacology
MH  - Monocytes
MH  - Muscle, Smooth, Vascular/*metabolism
MH  - Rabbits
EDAT- 2000/05/16 09:00
MHDA- 2000/06/24 11:00
CRDT- 2000/05/16 09:00
PHST- 2000/05/16 09:00 [pubmed]
PHST- 2000/06/24 11:00 [medline]
PHST- 2000/05/16 09:00 [entrez]
AID - 10.1161/01.atv.20.5.1293 [doi]
PST - ppublish
SO  - Arterioscler Thromb Vasc Biol. 2000 May;20(5):1293-300. doi: 
      10.1161/01.atv.20.5.1293.