PMID- 10810185
OWN - NLM
STAT- MEDLINE
DCOM- 20001010
LR  - 20131121
IS  - 0006-2979 (Print)
IS  - 0006-2979 (Linking)
VI  - 65
IP  - 4
DP  - 2000 Apr
TI  - Participation of glyceraldehyde-3-phosphate dehydrogenase in the regulation of 
      2,3-diphosphoglycerate level in erythrocytes.
PG  - 463-8
AB  - Data are presented concerning the possible participation of 
      glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in regulation of the glycolytic 
      pathway and the level of 2,3-diphosphoglycerate in erythrocytes. Experimental 
      support has been obtained for the hypothesis according to which a mild oxidation 
      of GAPDH must result in acceleration of glycolysis and in decrease in the level 
      of 2, 3-diphosphoglycerate due to the acyl phosphatase activity of the mildly 
      oxidized enzyme. Incubation of erythrocytes in the presence of 1 mM hydrogen 
      peroxide decreases 2,3-diphosphoglycerate concentration and causes accumulation 
      of 3-phosphoglycerate. It is assumed that the acceleration of glycolysis in the 
      presence of oxidative agents described previously by a number of authors could be 
      attributed to the acyl phosphatase activity of GAPDH. A pH-dependent complexing 
      of GAPDH and 3-phosphoglycerate kinase or 2, 3-diphosphoglycerate mutase is found 
      to determine the fate of 1,3-diphosphoglycerate that serves as a substrate for 
      the synthesis of 2,3-diphosphoglycerate as well as for the 3-phosphoglycerate 
      kinase reaction in glycolysis. A withdrawal of the two-enzyme complexes from the 
      erythrocyte lysates using Sepharose-bound anti-GAPDH antibodies prevents the 
      pH-dependent accumulation of the metabolites. The role of GAPDH in the regulation 
      of glycolysis and the level of 2,3-diphosphoglycerate in erythrocytes is 
      discussed.
FAU - Fokina, K V
AU  - Fokina KV
AD  - Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State 
      University, Moscow, 119899, Russia.
FAU - Yazykova, M Y
AU  - Yazykova MY
FAU - Danshina, P V
AU  - Danshina PV
FAU - Schmalhausen, E V
AU  - Schmalhausen EV
FAU - Muronetz, V I
AU  - Muronetz VI
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochemistry (Mosc)
JT  - Biochemistry. Biokhimiia
JID - 0376536
RN  - 138-81-8 (2,3-Diphosphoglycerate)
RN  - 9012-36-6 (Sepharose)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
RN  - EC 2.7.2.3 (Phosphoglycerate Kinase)
RN  - EC 5.4.2.4 (Bisphosphoglycerate Mutase)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - 2,3-Diphosphoglycerate/*blood
MH  - Animals
MH  - Bisphosphoglycerate Mutase/metabolism
MH  - Erythrocytes/*enzymology
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/*metabolism
MH  - Glycolysis
MH  - Humans
MH  - Hydrogen Peroxide/pharmacology
MH  - Hydrogen-Ion Concentration
MH  - Mice
MH  - Oxygen/metabolism
MH  - Phosphoglycerate Kinase/metabolism
MH  - Rabbits
MH  - Sepharose/metabolism
MH  - Substrate Specificity
MH  - Time Factors
EDAT- 2000/05/16 09:00
MHDA- 2000/10/14 11:01
CRDT- 2000/05/16 09:00
PHST- 2000/05/16 09:00 [pubmed]
PHST- 2000/10/14 11:01 [medline]
PHST- 2000/05/16 09:00 [entrez]
AID - BCM65040547 [pii]
PST - ppublish
SO  - Biochemistry (Mosc). 2000 Apr;65(4):463-8.