PMID- 10814786
OWN - NLM
STAT- MEDLINE
DCOM- 20000626
LR  - 20220310
IS  - 0165-5728 (Print)
IS  - 0165-5728 (Linking)
VI  - 106
IP  - 1-2
DP  - 2000 Jul 1
TI  - Inducible expression of the long pentraxin PTX3 in the central nervous system.
PG  - 87-94
AB  - PTX3 is a prototypic long pentraxin consisting of a C terminal 203-amino acid 
      pentraxin-like domain coupled with an N-terminal 178-amino acid unrelated 
      portion. PTX3 is induced by primary proinflammatory signals in various cell 
      types, most prominently macrophages and endothelial cells. Other long pentraxins, 
      such as murine or rat neuronal pentraxin 1 (NP1) and human neuronal pentraxin 2 
      (NPTX2), are expressed in the central nervous system (CNS). The present study was 
      designed to investigate whether PTX3 is expressed in the brain and to define the 
      structures and cells involved. Intracerebroventricular (i.c.v.), but not i.v., 
      injection of LPS induced high levels of PTX3 mRNA in the mouse brain. In contrast 
      NP1 is constitutively expressed in the murine CNS and is not modulated by LPS 
      administration. I.c.v. IL-1beta was also a potent inducer of PTX3 expression in 
      the CNS, whereas TNFalpha was substantially less effective and IL-6 induced a 
      barely detectable signal. Central administration of LPS and IL-1 induced PTX3 
      also in the periphery (heart), whereas the reverse did not occur. Expression of 
      PTX3 was also observed in the brain of mice infected with Candida albicans (C. 
      albicans) or Cryptococcus neoformans. (C. neoformans). The kinetics of PTX3 gene 
      induction were consistently different between C. albicans- and C. 
      neoformans-infected mice, according to the diverse outcome of the CNS immune 
      reaction. In situ hybridization revealed that i.c.v. injection of LPS induced a 
      strong PTX3 expression in presumptive glial cells, in the white matter (corpus 
      callosum, fimbria) and meningeal pia mater as well as in dentate gyrus hilus and 
      granule cells. No constitutive expression of PTX3 was detected. Central 
      expression of PTX3 may amplify mechanisms of innate resistance and damage in the 
      CNS. The possibility of a direct interaction of PTX3 with neuronal cells, as 
      suggested for NPTX2, remains to be explored.
FAU - Polentarutti, N
AU  - Polentarutti N
AD  - Istituto di Ricerche Farmacologiche 'Mario Negri', Via Eritrea 62, 20157, Milano, 
      Italy.
FAU - Bottazzi, B
AU  - Bottazzi B
FAU - Di Santo, E
AU  - Di Santo E
FAU - Blasi, E
AU  - Blasi E
FAU - Agnello, D
AU  - Agnello D
FAU - Ghezzi, P
AU  - Ghezzi P
FAU - Introna, M
AU  - Introna M
FAU - Bartfai, T
AU  - Bartfai T
FAU - Richards, G
AU  - Richards G
FAU - Mantovani, A
AU  - Mantovani A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Neuroimmunol
JT  - Journal of neuroimmunology
JID - 8109498
RN  - 0 (Interleukin-1)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Serum Amyloid P-Component)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (neuronal pentraxin)
RN  - 148591-49-5 (PTX3 protein)
RN  - 9007-41-4 (C-Reactive Protein)
SB  - IM
MH  - Animals
MH  - Brain/cytology/drug effects/*metabolism
MH  - C-Reactive Protein/genetics/*metabolism
MH  - Candidiasis/metabolism
MH  - Cryptococcosis/metabolism
MH  - Gene Expression Regulation
MH  - Humans
MH  - Injections, Intraventricular
MH  - Interleukin-1/pharmacology
MH  - Lipopolysaccharides/pharmacology
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Myocardium/metabolism
MH  - Nerve Tissue Proteins/metabolism
MH  - RNA, Messenger/metabolism
MH  - Recombinant Proteins/pharmacology
MH  - Serum Amyloid P-Component/genetics/*metabolism
MH  - Transcriptional Activation
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 2000/05/18 09:00
MHDA- 2000/07/06 11:00
CRDT- 2000/05/18 09:00
PHST- 2000/05/18 09:00 [pubmed]
PHST- 2000/07/06 11:00 [medline]
PHST- 2000/05/18 09:00 [entrez]
AID - S0165572800002149 [pii]
AID - 10.1016/s0165-5728(00)00214-9 [doi]
PST - ppublish
SO  - J Neuroimmunol. 2000 Jul 1;106(1-2):87-94. doi: 10.1016/s0165-5728(00)00214-9.