PMID- 10814854
OWN - NLM
STAT- MEDLINE
DCOM- 20000717
LR  - 20190819
IS  - 0300-483X (Print)
IS  - 0300-483X (Linking)
VI  - 146
IP  - 2-3
DP  - 2000 May 5
TI  - Comparison of mouse strains using the local lymph node assay.
PG  - 221-7
AB  - The local lymph node assay (LLNA), as recommended by the Interagency Coordinating 
      Committee on the Validation of Alternative Methods (ICCVAM), only allows for the 
      use of CBA mice. The objective of these studies was to begin to assess the 
      response of chemical sensitizers in the LLNA across six strains of female mice 
      (C57BL/6, SJL/J, BALB/c, B6C3F1, DBA/2 and CBA). The moderate sensitizer 
      alpha-hexylcinnamaldehyde (HCA) was chosen as the test chemical, while toluene 
      diisocyanate (TDI) and 2,4-dinitrofluorobenzene (DNFB) were evaluated at single 
      concentrations as positive controls. Draining lymph node cell proliferation 
      following acetone exposure varied across strains. SJL mice had a significantly 
      higher degree of proliferation with 2111 d.p.m./2 nodes. The remaining five 
      strains demonstrated responses which ranged from 345 to 887 dpm/2 nodes. DBA/2, 
      B6C3F1, BALB/c and CBA mice had essentially equal levels of lymph node 
      proliferation following exposure to the three chemicals. While C57BL/6 mice gave 
      similar results as CBA mice following DNFB and HCA administration, the LLNA 
      response to TDI was considerably lower. SJL mice provided low stimulation indexes 
      (SI) values for all three chemicals evaluated. Regardless of the level of LLNA 
      response, all six mouse strains identified the sensitization potential of HCA, 
      TDI or DNFB. Based on these studies, DBA/2, B6C3F1 and BALB/c mice are good 
      choices for continued evaluation as additional mouse strains for use in the LLNA.
FAU - Woolhiser, M R
AU  - Woolhiser MR
AD  - Health Effects Laboratory Division, National Institute for Occupational Safety 
      and Health, 1095 Willowdale Road, Morgantown, WV 26505, USA.
FAU - Munson, A E
AU  - Munson AE
FAU - Meade, B J
AU  - Meade BJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Ireland
TA  - Toxicology
JT  - Toxicology
JID - 0361055
RN  - 101-86-0 (hexyl cinnamic aldehyde)
RN  - 17X7AFZ1GH (Toluene 2,4-Diisocyanate)
RN  - 7864XYD3JJ (Acrolein)
RN  - D241E059U6 (Dinitrofluorobenzene)
RN  - VC2W18DGKR (Thymidine)
SB  - IM
MH  - Acrolein/analogs & derivatives/toxicity
MH  - Animals
MH  - Dermatitis, Allergic Contact/pathology
MH  - Dinitrofluorobenzene/toxicity
MH  - Female
MH  - Lymph Nodes/cytology/*drug effects
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Species Specificity
MH  - Stimulation, Chemical
MH  - Thymidine/metabolism
MH  - Toluene 2,4-Diisocyanate/toxicity
EDAT- 2000/05/18 09:00
MHDA- 2000/07/25 11:00
CRDT- 2000/05/18 09:00
PHST- 2000/05/18 09:00 [pubmed]
PHST- 2000/07/25 11:00 [medline]
PHST- 2000/05/18 09:00 [entrez]
AID - S0300483X00001529 [pii]
AID - 10.1016/s0300-483x(00)00152-9 [doi]
PST - ppublish
SO  - Toxicology. 2000 May 5;146(2-3):221-7. doi: 10.1016/s0300-483x(00)00152-9.