PMID- 10816528
OWN - NLM
STAT- MEDLINE
DCOM- 20000623
LR  - 20231213
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 68
IP  - 6
DP  - 2000 Jun
TI  - Listeria monocytogenes-infected human dendritic cells: uptake and host cell 
      response.
PG  - 3680-8
AB  - Dendritic cells (DCs) are potent antigen-presenting cells and play a crucial role 
      in initiation and modulation of specific immune responses. Various pathogens are 
      able to persist inside DCs. However, internalization of the gram-positive 
      bacterium Listeria monocytogenes into human DCs has not yet been shown. In the 
      present study, we demonstrate that human monocyte-derived immature DCs can 
      efficiently phagocytose L. monocytogenes. This uptake is independent of listerial 
      adhesion factors internalin A and internalin B but requires cytoskeletal motion 
      and factors present in human plasma. A major portion of internalized bacteria is 
      found in membrane-bound phagosomes and is rarely free in the cytosol, as shown by 
      transmission electron microscopy and by using an L. monocytogenes strain 
      expressing green fluorescent protein when in the host cell cytosol. The infection 
      caused maturation of the immature DCs into mature DCs displaying high levels of 
      CD83, CD25, major histocompatibility complex class II, and the CD86 costimulator 
      molecule. This effect appeared to be largely mediated by listerial lipoteichoic 
      acid. Although L. monocytogenes infection is known to induce death in other cell 
      types, infection of human DCs was found to induce necrotic but not apoptotic 
      death in fewer than 20% of DCs. Therefore, the ability of DCs to act as effective 
      antigen-presenting cells for listerial immunity is probably enhanced by their 
      resistance to cell death, as well as their ability to rapidly differentiate into 
      mature, immunostimulatory DCs upon encountering bacteria.
FAU - Kolb-Maurer, A
AU  - Kolb-Maurer A
AD  - Lehrstuhl fur Mikrobiologie, Theodor-Boveri-Institut fur Biowissenschaften der 
      Universitat Wurzburg, 97074 Wurzburg, Germany.ankolb@biozentrum.uniwuerzburg.de
FAU - Gentschev, I
AU  - Gentschev I
FAU - Fries, H W
AU  - Fries HW
FAU - Fiedler, F
AU  - Fiedler F
FAU - Brocker, E B
AU  - Brocker EB
FAU - Kampgen, E
AU  - Kampgen E
FAU - Goebel, W
AU  - Goebel W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Antigens, CD)
RN  - 0 (B7-2 Antigen)
RN  - 0 (CD86 protein, human)
RN  - 0 (Cytokines)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Immunoglobulins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Interleukin-2)
SB  - IM
MH  - Antigens, CD/isolation & purification
MH  - B7-2 Antigen
MH  - Blood Cells
MH  - Cell Differentiation
MH  - Cell Separation
MH  - Cytokines/pharmacology
MH  - Dendritic Cells/drug effects/*immunology/microbiology
MH  - Hemolysin Factors/genetics
MH  - Histocompatibility Antigens Class II/isolation & purification
MH  - Humans
MH  - Immunoglobulins/isolation & purification
MH  - Listeria monocytogenes/*immunology/pathogenicity
MH  - Membrane Glycoproteins/isolation & purification
MH  - Mutation
MH  - Necrosis
MH  - *Phagocytosis/drug effects
MH  - Phagosomes/microbiology
MH  - Receptors, Interleukin-2/isolation & purification
MH  - CD83 Antigen
PMC - PMC97659
EDAT- 2000/05/19 09:00
MHDA- 2000/07/06 11:00
PMCR- 2000/06/01
CRDT- 2000/05/19 09:00
PHST- 2000/05/19 09:00 [pubmed]
PHST- 2000/07/06 11:00 [medline]
PHST- 2000/05/19 09:00 [entrez]
PHST- 2000/06/01 00:00 [pmc-release]
AID - 1894 [pii]
AID - 10.1128/IAI.68.6.3680-3688.2000 [doi]
PST - ppublish
SO  - Infect Immun. 2000 Jun;68(6):3680-8. doi: 10.1128/IAI.68.6.3680-3688.2000.