PMID- 10818209
OWN - NLM
STAT- MEDLINE
DCOM- 20000728
LR  - 20161018
IS  - 0938-8990 (Print)
IS  - 0938-8990 (Linking)
VI  - 11
IP  - 6
DP  - 2000 Jun
TI  - Isolation, characterization, expression and functional analysis of the zebrafish 
      ortholog of MEN1.
PG  - 448-54
AB  - Mutations in the MEN1 gene lead to an autosomal dominant disorder, multiple 
      endocrine neoplasia type 1 (MEN1), which is characterized by tumors of the 
      parathyroid, entero-pancreatic neuroendocrine, and pituitary tissues. The protein 
      encoded by MEN1, 610-amino acid menin, resides primarily in the nucleus and binds 
      to the transcription factor JunD, resulting in the repression of JunD-induced 
      transcription. We report here a detailed characterization of the zebrafish men1 
      gene and its full-length (2551 nt) transcript, encoding a 617-amino acid protein 
      with 67% identity and 80% similarity to human menin. Of the 81 missense mutations 
      and in-frame deletions reported in MEN1 patients, 72 occur in residues that are 
      identical in zebrafish, suggesting the importance of the conserved regions. The 
      zebrafish men1 gene maps 61 cM from the top of linkage group 7 (LG7), a region 
      that appears to show conserved synteny to the MEN1 loci at human 11q13. A 2.7-kb 
      men1 message is detected at all stages of zebrafish development analyzed, from 
      one-cell embryos to adult fish. Whole-mount in situ hybridization showed 
      ubiquitous distribution of men1 message in zebrafish embryos at cleavage, 
      blastula, gastrula, and early segmentation stages, with relatively abundant 
      expression in blood cell progenitors (24 h post fertilization) and mesenchymal 
      tissues (48 h post fertilization) at later stages. Zebrafish menin binds both 
      human and mouse JunD, and represses JunD-induced transcription, indicating that 
      the JunD-binding ability of menin is evolutionarily conserved.
FAU - Manickam, P
AU  - Manickam P
AD  - Genetics and Molecular Biology Branch, National Human Genome Research Institute, 
      National Institutes of Health, Bldg. 49, Rm. 3E13, 49 Convent Drive, Bethesda, 
      Maryland 20892-4442, USA.
FAU - Vogel, A M
AU  - Vogel AM
FAU - Agarwal, S K
AU  - Agarwal SK
FAU - Oda, T
AU  - Oda T
FAU - Spiegel, A M
AU  - Spiegel AM
FAU - Marx, S J
AU  - Marx SJ
FAU - Collins, F S
AU  - Collins FS
FAU - Weinstein, B M
AU  - Weinstein BM
FAU - Chandrasekharappa, S C
AU  - Chandrasekharappa SC
LA  - eng
SI  - GENBANK/AF212919
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mamm Genome
JT  - Mammalian genome : official journal of the International Mammalian Genome Society
JID - 9100916
RN  - 0 (DNA, Complementary)
RN  - 0 (MEN1 protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 63231-63-0 (RNA)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Chromosome Mapping
MH  - DNA/chemistry/genetics
MH  - DNA, Complementary/chemistry/*genetics/isolation & purification
MH  - Embryo, Nonmammalian/metabolism
MH  - Embryonic Development
MH  - Exons
MH  - Gene Expression
MH  - Gene Expression Regulation, Developmental
MH  - Humans
MH  - In Situ Hybridization
MH  - Introns
MH  - Mice
MH  - Molecular Sequence Data
MH  - Neoplasm Proteins/*genetics/metabolism
MH  - Protein Binding
MH  - *Proto-Oncogene Proteins
MH  - Proto-Oncogene Proteins c-jun/genetics/metabolism
MH  - RNA/genetics/metabolism
MH  - Rats
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Transcriptional Activation
MH  - Zebrafish/embryology/*genetics
EDAT- 2000/05/20 09:00
MHDA- 2000/08/06 11:00
CRDT- 2000/05/20 09:00
PHST- 2000/05/20 09:00 [pubmed]
PHST- 2000/08/06 11:00 [medline]
PHST- 2000/05/20 09:00 [entrez]
AID - MG00-806 [pii]
AID - 10.1007/s003350010085 [doi]
PST - ppublish
SO  - Mamm Genome. 2000 Jun;11(6):448-54. doi: 10.1007/s003350010085.