PMID- 10819523
OWN - NLM
STAT- MEDLINE
DCOM- 20000606
LR  - 20191103
IS  - 1083-3021 (Print)
IS  - 1083-3021 (Linking)
VI  - 3
IP  - 2
DP  - 1998 Apr
TI  - Roles of hepatocyte growth factor/scatter factor and transforming growth 
      factor-beta1 in mammary gland ductal morphogenesis.
PG  - 133-50
AB  - Epithelial-mesenchymal interactions are responsible for the unique pattern of 
      ductal branching morphogenesis characteristic of the mammary gland. To 
      investigate the factors which control the elongation and branching of lactiferous 
      ducts, we developed an in vitro model of ductal morphogenesis in which clonal 
      mouse mammary epithelial cells (TAC-2 cells) are grown in collagen gels. In this 
      experimental system, fibroblast conditioned medium (CM)3 stimulates the formation 
      of extensively arborized tubules. The molecule responsible for this tubulogenic 
      effect was identified as hepatocyte growth factor/scatter factor (HGF/SF). To 
      determine whether HGF/SF plays a role in mammary gland morphogenesis in vivo, the 
      expression of HGF/SF and its receptor, c-Met, were analyzed in the rat mammary 
      gland during pregnancy, lactation, and involution. Levels of HGF/SF and c-Met 
      transcripts were progressively reduced during pregnancy, were virtually 
      undetectable during lactation, and increased again during involution. 
      Collectively, these in vitro and in vivo findings suggest that HGF/SF is a 
      paracrine mediator of mammary gland ductal morphogenesis. We subsequently 
      investigated the effect of another multifunctional cytokine, namely TGF-beta1, on 
      branching morphogenesis of TAC-2 cells. TGF-beta1 had a striking biphasic effect: 
      whereas relatively high concentrations of this cytokine inhibited colony 
      formation, lower concentrations stimulated extensive elongation and branching of 
      epithelial cords. Taken together, these studies indicate that HGF/SF is a 
      stromal-derived paracrine mediator of mammary ductal morphogenesis, and that when 
      present at low concentrations, TGF-beta1 can contribute to this process.
FAU - Soriano, J V
AU  - Soriano JV
AD  - Department of Morphology, University of Geneva Medical School, Switzerland. 
      Jesus.SorianoMolla@medecine.unige.ch
FAU - Pepper, M S
AU  - Pepper MS
FAU - Orci, L
AU  - Orci L
FAU - Montesano, R
AU  - Montesano R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - United States
TA  - J Mammary Gland Biol Neoplasia
JT  - Journal of mammary gland biology and neoplasia
JID - 9601804
RN  - 0 (Transforming Growth Factor beta)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Animals
MH  - Breast/*embryology/*growth & development
MH  - Cell Line
MH  - Epithelial Cells/cytology/physiology
MH  - Female
MH  - Hepatocyte Growth Factor/*physiology
MH  - Humans
MH  - Lactation
MH  - Mammary Glands, Animal/cytology/*embryology/*growth & development
MH  - Mice
MH  - Morphogenesis
MH  - Pregnancy
MH  - Rats
MH  - Transforming Growth Factor beta/*physiology
RF  - 92
EDAT- 2000/05/20 09:00
MHDA- 2000/06/10 09:00
CRDT- 2000/05/20 09:00
PHST- 2000/05/20 09:00 [pubmed]
PHST- 2000/06/10 09:00 [medline]
PHST- 2000/05/20 09:00 [entrez]
AID - 10.1023/a:1018790705727 [doi]
PST - ppublish
SO  - J Mammary Gland Biol Neoplasia. 1998 Apr;3(2):133-50. doi: 
      10.1023/a:1018790705727.