PMID- 10819779
OWN - NLM
STAT- MEDLINE
DCOM- 20000817
LR  - 20220321
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 62
IP  - 6
DP  - 2000 Jun
TI  - Involvement of mitochondria in oxidative stress-induced cell death in mouse 
      zygotes.
PG  - 1745-53
AB  - Accumulation of reactive oxygen species during aging leads to programmed cell 
      death (PCD) in many cell types but has not been explored in mammalian fertilized 
      eggs, in which mitochondria are "immature," in contrast to "mature" mitochondria 
      in somatic cells. We characterized PCD in mouse zygotes induced by either 
      intensive (1 mM for 1.5 h) or mild (200 microM for 15 min) hydrogen peroxide 
      (H(2)O(2)) treatment. Shortly after intensive treatment, zygotes displayed PCD, 
      typified by cell shrinkage, cytochrome c release from mitochondria, and caspase 
      activation, then terminal deoxynucleotidyl transferase-mediated dUTP nick end 
      labeling (TUNEL) staining in condensed pronuclei. On the other hand, after mild 
      treatment, zygotes arrested developmentally and showed neither cytochrome c 
      release nor caspase activation over 48 h; until 72 h, 46% zygotes exhibited TUNEL 
      staining, and 88% of zygotes lost plasma membrane integrity. Interestingly, mild 
      oxidative treatment induced a decline in mitochondrial membrane potential and 
      disruption of the mitochondrial matrix. Taken together, these results suggest 
      that oxidative stress caused by H(2)O(2) induces PCD in mouse zygotes and that 
      mitochondria are involved in the early phase of oxidative stress-induced PCD. 
      Furthermore, mitochondrial malfunction also may contribute to cell cycle arrest, 
      followed by cell death, triggered by mild oxidative stress.
FAU - Liu, L
AU  - Liu L
AD  - Department of Obstetrics & Gynaecology, Women & Infants Hospital, Brown 
      University, Providence, Rhode Island 02905, USA.
FAU - Trimarchi, J R
AU  - Trimarchi JR
FAU - Keefe, D L
AU  - Keefe DL
LA  - eng
GR  - K081099/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Cytochrome c Group)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Caspases/metabolism
MH  - Cell Nucleus/ultrastructure
MH  - Cytochrome c Group/metabolism
MH  - Enzyme Activation
MH  - Female
MH  - Hydrogen Peroxide/pharmacology
MH  - In Situ Nick-End Labeling
MH  - Membrane Potentials
MH  - Mice
MH  - Mitochondria/*physiology
MH  - *Oxidative Stress
MH  - Zygote/*ultrastructure
EDAT- 2000/05/20 09:00
MHDA- 2000/08/19 11:00
CRDT- 2000/05/20 09:00
PHST- 2000/05/20 09:00 [pubmed]
PHST- 2000/08/19 11:00 [medline]
PHST- 2000/05/20 09:00 [entrez]
AID - 10.1095/biolreprod62.6.1745 [doi]
PST - ppublish
SO  - Biol Reprod. 2000 Jun;62(6):1745-53. doi: 10.1095/biolreprod62.6.1745.