PMID- 10821492
OWN - NLM
STAT- MEDLINE
DCOM- 20000601
LR  - 20190722
IS  - 0046-8177 (Print)
IS  - 0046-8177 (Linking)
VI  - 31
IP  - 4
DP  - 2000 Apr
TI  - Malignant cell detection by fluorescence in situ hybridization (FISH) in 
      effusions from patients with carcinoma.
PG  - 448-55
AB  - Cytological diagnosis of malignant cells in effusions is hampered by difficulties 
      in the differentiation from reactive mesothelial cells. Because interphase 
      cytogenetics by fluorescence in situ hybridization (FISH) might complement 
      cytological evaluation, we determined the power of tumor cell detection using 
      FISH and cytology in 201 effusions from patients with advanced cancer. 
      Furthermore, 9 primary breast tumors were FISH-karyotyped, and chromosomal 
      aberrations were compared with those of corresponding metastatic effusion cells. 
      By using centromeric probes representing chromosomes 7, 8, 11, 12, 17, and 18, a 
      rate of malignancy-associated aneusomy combined for the 6 chromosomes was 
      detected in an overall of 44.8% of effusion specimens (range, 31.8% to 39.3% for 
      the individual chromosome), comparable to cytology (43.3%). The combination of 
      just 2 FISH probes (namely, representing chromosome pairs 8/11 and 8/17) was 
      almost equally efficient in the identification of aneusomy. Approximately one 
      fourth of the cytologically negative effusions were FISH positive and vice versa. 
      From the initially FISH-negative effusions, 18.9% could be subsequently 
      classified positive with dual-color FISH by visualization of intranuclear 
      chromosomal complexity in rare aneuploid cells. Thus, "overall FISH analysis," 
      including dual-color evaluation, identified tumor cells in significantly more 
      effusions (55.2%, P = .001) than conventional cytology, implying greater 
      sensitivity. Finally, our finding that numerical aberration patterns in primary 
      breast tumors and corresponding metastatic effusions are comparable indicates 
      that FISH examination of primary tumors will indicate the centromeric probe(s) 
      best suited for an efficient search for metastasis in the individual case.
FAU - Fiegl, M
AU  - Fiegl M
AD  - First Department of Internal Medicine, University of Vienna, Austria.
FAU - Kaufmann, H
AU  - Kaufmann H
FAU - Zojer, N
AU  - Zojer N
FAU - Schuster, R
AU  - Schuster R
FAU - Wiener, H
AU  - Wiener H
FAU - Mullauer, L
AU  - Mullauer L
FAU - Roka, S
AU  - Roka S
FAU - Huber, H
AU  - Huber H
FAU - Drach, J
AU  - Drach J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hum Pathol
JT  - Human pathology
JID - 9421547
SB  - IM
MH  - Adenocarcinoma/genetics/*secondary
MH  - Aneuploidy
MH  - Ascitic Fluid/genetics/*pathology
MH  - Breast Neoplasms/genetics/pathology
MH  - Chromosomes, Human
MH  - Evaluation Studies as Topic
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Neoplasm Metastasis/diagnosis/genetics
MH  - Neoplasms/genetics/*pathology
MH  - Pleural Effusion, Malignant/genetics/*pathology
EDAT- 2000/05/23 09:00
MHDA- 2000/06/03 09:00
CRDT- 2000/05/23 09:00
PHST- 2000/05/23 09:00 [pubmed]
PHST- 2000/06/03 09:00 [medline]
PHST- 2000/05/23 09:00 [entrez]
AID - S0046-8177(05)80030-9 [pii]
AID - 10.1053/hp.2000.6550 [doi]
PST - ppublish
SO  - Hum Pathol. 2000 Apr;31(4):448-55. doi: 10.1053/hp.2000.6550.