PMID- 10822021
OWN - NLM
STAT- MEDLINE
DCOM- 20000719
LR  - 20190831
IS  - 0960-0760 (Print)
IS  - 0960-0760 (Linking)
VI  - 73
IP  - 1-2
DP  - 2000 May
TI  - Absence of correlation between in situ expression of cytochrome P450 17alpha 
      hydroxylase/lyase and 3beta-hydroxysteroid dehydrogenase/(Delta5-4) isomerase 
      messenger ribonucleic acids and steroidogenesis during pubertal development in 
      the rat testis.
PG  - 19-28
AB  - Changes in expression of Leydig cell 3beta-hydroxysteroid dehydrogenase 
      (3betaHSD) and 17alpha-hydroxylase/C17-20 lyase (P450(17alpha)) messenger RNA 
      (mRNA) during pubertal development have not been well characterized in the rat. 
      In the present study, expression of 3betaHSD and P450(17alpha) were determined in 
      frozen sections of testes of immature (days 21 and 28), pubertal (days 45 and 60) 
      and adult (day 90) rats by in situ hybridization using digoxigenin-labeled 
      riboprobes and quantified densitometrically. Measures of steroidogenesis in this 
      study, 3betaHSD and P450(17alpha) enzyme activities per testis and plasma 
      testosterone concentration, increased during pubertal development, peaking at 
      45-60 days of age. Expression of 3betaHSD protein, a marker for Leydig cell 
      function, was abundantly immunolocalized to the interstitial compartment of the 
      testis. Quantified densitometrically, the amount of 3betaHSD protein did not vary 
      significantly during pubertal development. Transcripts of 3betaHSD and 
      P450(17alpha) were expressed abundantly by clusters of immature Leydig cells in 
      immature animals. However, in contrast to measures of steroidogenesis during 
      pubertal development, mRNA of 3betaHSD and P450(17alpha) decreased to 
      undetectable levels at the age of 45 and 60 days, respectively. The decline in 
      mRNA of 3betaHSD and P450(17alpha) was confirmed by Northern analysis. Expression 
      of 3betaHSD and P450(17alpha) transcripts rebounded in the adult at 90 days and 
      were comparable to levels of expression observed in immature animals. These 
      results show that during pubertal development the steady-state accumulation of 
      mRNA of 3betaHSD and P450(17alpha) are not correlated with accumulation of 
      3betaHSD protein, enzyme activities of 3betaHSD and P450(17alpha), or 
      testosterone secretion. Possible explanations of the depletion of transcripts 
      during pubertal development include: specific inhibition of transcription, 
      increased mRNA instability, or high translational activity.
FAU - Mack, S O
AU  - Mack SO
AD  - Department of Physiology and Biophysics, Howard University College of Medicine, 
      Washington, DC 20059, USA. smack@fac.howard.edu
FAU - Garrett, W M
AU  - Garrett WM
FAU - Guthrie, H D
AU  - Guthrie HD
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Steroid Biochem Mol Biol
JT  - The Journal of steroid biochemistry and molecular biology
JID - 9015483
RN  - 0 (3 beta-hydroxysteroid oxidoreductase-delta(5) 3-ketosteroid isomerase)
RN  - 0 (Androgens)
RN  - 0 (Multienzyme Complexes)
RN  - 0 (RNA, Messenger)
RN  - 3XMK78S47O (Testosterone)
RN  - EC 1.1.1.145 (Progesterone Reductase)
RN  - EC 1.14.14.19 (Steroid 17-alpha-Hydroxylase)
RN  - EC 5.3.3.- (Steroid Isomerases)
SB  - IM
MH  - Androgens/*biosynthesis
MH  - Animals
MH  - Gene Expression Regulation, Developmental
MH  - Gene Expression Regulation, Enzymologic
MH  - In Situ Hybridization
MH  - Leydig Cells/metabolism
MH  - Male
MH  - Multienzyme Complexes/*genetics
MH  - Progesterone Reductase/*genetics
MH  - RNA, Messenger/*genetics/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sexual Maturation
MH  - Steroid 17-alpha-Hydroxylase/*genetics
MH  - Steroid Isomerases/*genetics
MH  - Testis/*growth & development/*metabolism
MH  - Testosterone/blood
EDAT- 2000/05/24 09:00
MHDA- 2000/07/25 11:00
CRDT- 2000/05/24 09:00
PHST- 2000/05/24 09:00 [pubmed]
PHST- 2000/07/25 11:00 [medline]
PHST- 2000/05/24 09:00 [entrez]
AID - S0960-0760(00)00048-0 [pii]
AID - 10.1016/s0960-0760(00)00048-0 [doi]
PST - ppublish
SO  - J Steroid Biochem Mol Biol. 2000 May;73(1-2):19-28. doi: 
      10.1016/s0960-0760(00)00048-0.