PMID- 10822165
OWN - NLM
STAT- MEDLINE
DCOM- 20000717
LR  - 20190719
IS  - 0361-9230 (Print)
IS  - 0361-9230 (Linking)
VI  - 52
IP  - 3
DP  - 2000 Jun
TI  - Brain interleukin-1beta and tumor necrosis factor-alpha are involved in 
      lipopolysaccharide-induced delayed rectal allodynia in awake rats.
PG  - 223-8
AB  - Recently, we have developed a model of delayed (12 h) increase in sensitivity 
      (allodynia) to rectal distension (RD) induced by intraperitoneal 
      lipopolysaccharide (LPS) in awake rats. Thus, we examined whether central 
      interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha) are 
      involved in LPS response. Abdominal contractions (criterion of visceral pain) 
      were recorded in rats equipped with intramuscular electrodes. RDs were performed 
      at various times after pharmacological treatments. RD induced abdominal 
      contractions from a threshold volume of distension of 0.8 ml. At lowest volume 
      (0.4 ml), this number was significantly increased 12 h after LPS. 
      Intracerebroventricular (i.c.v.) injection of IL-1 receptor antagonist, IL-1beta 
      converting enzyme inhibitor or recombinant human TNF-alpha soluble receptor 
      reduced LPS-induced increase of abdominal contractions at 0.4 ml volume of 
      distension. When injected i.c.v., recombinant human IL-1beta and recombinant 
      bovine TNF-alpha reproduced LPS response at 9 and 12 h and at 6 and 9 h, 
      respectively. These data suggest that IL-1beta and TNF-alpha act centrally to 
      induce delayed rectal hypersensitivity and that central release of these 
      cytokines is responsible of LPS-induced delayed (12 h) rectal allodynia.
FAU - Coelho, A
AU  - Coelho A
AD  - Institut National de la Recherche Agronomique, Neurogastroenterology and 
      Nutrition Unit, Toulouse, France.
FAU - Fioramonti, J
AU  - Fioramonti J
FAU - Bueno, L
AU  - Bueno L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Brain Res Bull
JT  - Brain research bulletin
JID - 7605818
RN  - 0 (Cysteine Proteinase Inhibitors)
RN  - 0 (Interleukin-1)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Serpins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Viral Proteins)
RN  - 96282-35-8 (interleukin-1beta-converting enzyme inhibitor)
SB  - IM
MH  - Animals
MH  - Brain/*metabolism
MH  - Consciousness
MH  - Cysteine Proteinase Inhibitors/pharmacology
MH  - Electromyography
MH  - Hyperalgesia/chemically induced/drug therapy/*metabolism
MH  - Injections, Intraventricular
MH  - Interleukin-1/*metabolism/pharmacology
MH  - Lipopolysaccharides/pharmacology
MH  - Male
MH  - Nociceptors/physiology
MH  - Pain Measurement
MH  - Rats
MH  - Rats, Wistar
MH  - Recombinant Proteins/pharmacology
MH  - Rectum/*innervation/*physiology
MH  - Serpins/pharmacology
MH  - Tumor Necrosis Factor-alpha/*metabolism/pharmacology
MH  - *Viral Proteins
EDAT- 2000/05/24 09:00
MHDA- 2000/07/25 11:00
CRDT- 2000/05/24 09:00
PHST- 2000/05/24 09:00 [pubmed]
PHST- 2000/07/25 11:00 [medline]
PHST- 2000/05/24 09:00 [entrez]
AID - S0361923000002690 [pii]
AID - 10.1016/s0361-9230(00)00269-0 [doi]
PST - ppublish
SO  - Brain Res Bull. 2000 Jun;52(3):223-8. doi: 10.1016/s0361-9230(00)00269-0.