PMID- 10824598
OWN - NLM
STAT- MEDLINE
DCOM- 20000608
LR  - 20190818
IS  - 0031-8655 (Print)
IS  - 0031-8655 (Linking)
VI  - 71
IP  - 4
DP  - 2000 Apr
TI  - Plasma membrane associated location of sulfonated meso-tetraphenylporphyrins of 
      different hydrophilicity probed by total internal reflection fluorescence 
      spectroscopy.
PG  - 460-5
AB  - Sulfonated meso-tetraphenylporphyrins of different hydrophilicity were 
      microspectrofluorimetrically examined in endothelial cells using total internal 
      reflection (TIR) illumination or epi-illumination. Since the penetration depth of 
      the evanescent field during TIR illumination is limited to a few hundred 
      nanometers, photosensitizers were almost selectively examined in close vicinity 
      to the plasma membrane. Pronounced fluorescence signals during TIR illumination 
      were observed for the hydrophilic compounds meso-tetraphenylporphyrin 
      tetrasulfonate (TPPS4) and meso-tetraphenylporphyrin trisulfonate (TPPS3), 
      whereas the more lipophilic compounds meso-tetraphenylporphyrin disulfonate 
      (TPPS2a) and meso-tetraphenylporphyrin monosulfonate (TPPS1) could only be 
      detected under epi-illumination. Irradiation of TPPS1 and TPPS2a in the Soret 
      band led to an increase in fluorescence intensity and formation of a photoproduct 
      with an emission maximum around 610 nm, which was limited to intracellular 
      compartments. In contrast, fluorescence spectra of TPPS3 and TPPS4 obtained by 
      TIR and epi-illumination remained almost unchanged after irradiation in the Soret 
      band. Extralysosomal location of TPPS3 and TPPS4 in close proximity to the plasma 
      membrane was deduced from experiments with the lysosomal markers acridine orange 
      (AO) or lysotracker yellow (LY), which were not detectable under TIR 
      illumination. In conclusion, these results provide for the first time direct 
      evidence for a plasma membrane-associated fraction of the hydrophilic compounds 
      TPPS3 and TPPS4 in living cells.
FAU - Sailer, R
AU  - Sailer R
AD  - Institut fur Lasertechnologien in der Medizin und Messtechnik, Universitat Ulm, 
      Germany. reinhard.sailer@ilm.uni-ulm.de
FAU - Strauss, W S
AU  - Strauss WS
FAU - Emmert, H
AU  - Emmert H
FAU - Stock, K
AU  - Stock K
FAU - Steiner, R
AU  - Steiner R
FAU - Schneckenburger, H
AU  - Schneckenburger H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Photochem Photobiol
JT  - Photochemistry and photobiology
JID - 0376425
RN  - 0 (Porphyrins)
SB  - IM
MH  - Cell Membrane/metabolism
MH  - Porphyrins/*metabolism
MH  - Spectrometry, Fluorescence
EDAT- 2000/05/29 09:00
MHDA- 2000/06/10 09:00
CRDT- 2000/05/29 09:00
PHST- 2000/05/29 09:00 [pubmed]
PHST- 2000/06/10 09:00 [medline]
PHST- 2000/05/29 09:00 [entrez]
AID - 10.1562/0031-8655(2000)071<0460:pmalos>2.0.co;2 [doi]
PST - ppublish
SO  - Photochem Photobiol. 2000 Apr;71(4):460-5. doi: 
      10.1562/0031-8655(2000)071<0460:pmalos>2.0.co;2.