PMID- 10824998
OWN - NLM
STAT- MEDLINE
DCOM- 20000720
LR  - 20191025
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 28
IP  - 2
DP  - 2000 Jun
TI  - MOZ is fused to p300 in an acute monocytic leukemia with t(8;22).
PG  - 138-44
AB  - We report on the fusion of the monocytic leukemia zinc finger protein (MOZ) gene 
      to the adenoviral E1A-associated protein p300 (p300) gene in acute monocytic 
      leukemia M5 associated with a t(8;22)(p11;q13) translocation. We studied two 
      patients with double-color fluorescence in situ hybridization (FISH) using the 
      yeast artificial chromosome 176C9 and the bacterial artificial chromosome clone 
      H59D10 specific to the MOZ and p300 genes, respectively. Both probes were split 
      in the patients' chromosome metaphase cells, and the two derivative chromosomes 
      were each labeled with both probes. We showed by Southern blot the rearrangement 
      of the MOZ gene, and cloned the fusion transcripts in one patient carrying the 
      t(8;22) by reverse transcription-polymerase chain reaction using MOZ- and 
      p300-specific primers. Both fusion transcripts were expressed. This result 
      defines a novel reciprocal translocation involving two acetyltransferases, MOZ 
      and p300, resulting in an abnormal transcriptional co-activator that could play a 
      critical role in leukemogenesis.
FAU - Chaffanet, M
AU  - Chaffanet M
AD  - Laboratoire d'Oncologie Moleculaire, INSERM U119, Marseille, France.
FAU - Gressin, L
AU  - Gressin L
FAU - Preudhomme, C
AU  - Preudhomme C
FAU - Soenen-Cornu, V
AU  - Soenen-Cornu V
FAU - Birnbaum, D
AU  - Birnbaum D
FAU - Pebusque, M J
AU  - Pebusque MJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Trans-Activators)
RN  - EC 2.3.1.- (Acetyltransferases)
RN  - EC 2.3.1.48 (E1A-Associated p300 Protein)
RN  - EC 2.3.1.48 (Histone Acetyltransferases)
RN  - EC 2.3.1.48 (KAT6A protein, human)
SB  - IM
MH  - Acetyltransferases/*genetics/isolation & purification
MH  - Amino Acid Sequence
MH  - Chromosomes, Human, Pair 22/*genetics
MH  - Chromosomes, Human, Pair 8/*genetics
MH  - E1A-Associated p300 Protein
MH  - Gene Rearrangement
MH  - Histone Acetyltransferases
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Monocytic, Acute/enzymology/*genetics
MH  - Leukemia, Myelomonocytic, Chronic/enzymology/genetics
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/*genetics/isolation & purification
MH  - RNA, Messenger/isolation & purification
MH  - Recombinant Fusion Proteins/genetics/isolation & purification
MH  - Trans-Activators/*genetics/isolation & purification
MH  - Translocation, Genetic/*genetics
MH  - Tumor Cells, Cultured
EDAT- 2000/05/29 09:00
MHDA- 2000/07/25 11:00
CRDT- 2000/05/29 09:00
PHST- 2000/05/29 09:00 [pubmed]
PHST- 2000/07/25 11:00 [medline]
PHST- 2000/05/29 09:00 [entrez]
AID - 10.1002/(SICI)1098-2264(200006)28:2<138::AID-GCC2>3.0.CO;2-2 [pii]
AID - 10.1002/(sici)1098-2264(200006)28:2<138::aid-gcc2>3.0.co;2-2 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2000 Jun;28(2):138-44. doi: 
      10.1002/(sici)1098-2264(200006)28:2<138::aid-gcc2>3.0.co;2-2.