PMID- 10825005
OWN - NLM
STAT- MEDLINE
DCOM- 20000720
LR  - 20061115
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 28
IP  - 2
DP  - 2000 Jun
TI  - Mapping the amplification of EIF3S3 in breast and prostate cancer.
PG  - 203-10
AB  - Gain of chromosome arm 8q is a frequent genetic alteration in breast and prostate 
      cancer. Two amplified subregions, 8q21 and 8q23-24, have been identified with 
      comparative genomic hybridization (CGH). We have recently demonstrated that the 
      EIF3S3 (eIF3-p40) gene, located at 8q23, is often amplified and overexpressed in 
      both breast and prostate cancer. Here, we used fluorescence in situ hybridization 
      (FISH) to map the amplified region around EIF3S3 in primary breast cancers and 
      cell lines. The size of the common highly amplified region was about 2.5 Mb 
      between the markers D8S1668 and WI-7959. Next, we analyzed the expression of all 
      expressed sequence tags (ESTs) located within and near this region by RNA slot 
      blot hybridization. In addition to EIF3S3, three anonymous ESTs and EXT1 were 
      found to be highly expressed in cancer cell lines with the amplification at 
      8q23-q24. However, the anonymous ESTs were located outside the minimal highly 
      amplified region and EXT1 was overexpressed only in one of the cancer cell lines 
      with 8q amplification. Since EIF3S3 was the only consistently overexpressed gene 
      located in the minimal highly amplified region, it is the strongest candidate 
      target gene for 8q23-q24 amplification.
FAU - Nupponen, N N
AU  - Nupponen NN
AD  - Laboratory of Cancer Genetics, Institute of Medical Technology, University of 
      Tampere and Tampere University Hospital, Finland.
FAU - Isola, J
AU  - Isola J
FAU - Visakorpi, T
AU  - Visakorpi T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Viral)
RN  - 0 (Eukaryotic Initiation Factor-3)
RN  - 0 (Genetic Markers)
RN  - 0 (Peptide Initiation Factors)
SB  - IM
MH  - Bacteriophages/genetics
MH  - Breast Neoplasms/*genetics
MH  - *Chromosome Mapping/methods
MH  - Chromosomes, Human, Pair 8/genetics
MH  - DNA Probes/genetics
MH  - DNA, Viral/genetics
MH  - Eukaryotic Initiation Factor-3
MH  - Expressed Sequence Tags
MH  - Female
MH  - Gene Amplification/*genetics
MH  - Gene Expression Profiling
MH  - Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Nucleic Acid Hybridization/methods
MH  - Peptide Initiation Factors/*genetics
MH  - Prostatic Neoplasms/*genetics
MH  - Tumor Cells, Cultured
EDAT- 2000/05/29 09:00
MHDA- 2000/07/25 11:00
CRDT- 2000/05/29 09:00
PHST- 2000/05/29 09:00 [pubmed]
PHST- 2000/07/25 11:00 [medline]
PHST- 2000/05/29 09:00 [entrez]
AID - 10.1002/(SICI)1098-2264(200006)28:2<203::AID-GCC9>3.0.CO;2-X [pii]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2000 Jun;28(2):203-10.