PMID- 10825227
OWN - NLM
STAT- MEDLINE
DCOM- 20000714
LR  - 20220316
IS  - 0021-9304 (Print)
IS  - 0021-9304 (Linking)
VI  - 51
IP  - 2
DP  - 2000 Aug
TI  - A biodegradable hybrid sponge nested with collagen microsponges.
PG  - 273-9
AB  - A biodegradable hybrid sponge of poly(DL-lactic-co-glycolic acid) (PLGA) and 
      collagen was fabricated by forming microsponges of collagen in the pores of PLGA 
      sponge. Observation of the PLGA-collagen hybrid sponge by scanning electron 
      microscopy (SEM) showed that microsponges of collagen with interconnected pore 
      structures were formed in the pores of PLGA sponge. The hybrid structure further 
      was confirmed by scanning electron microscopy-electron probe microanalysis 
      (SEM-EPMA), and elemental nitrogen was detected in the microsponges of collagen 
      and on the pore surfaces of PLGA, but not in cross-sections of PLGA regions. The 
      formation of collagen microsponges was dependent on collagen concentration, the 
      effective range of which was from 0.1 to 1.5 (w/v) %. The mechanical strength of 
      the hybrid sponge was higher than that of either PLGA or collagen sponges, in 
      both dry and wet states. The wettability with water was improved by hybridization 
      with collagen, which facilitated cell seeding in the hybrid sponge. Mouse 
      fibroblast L929 cells attached well and spread on the surfaces of the 
      microsponges of collagen in the hybrid sponge. The distribution of cells was 
      spatially uniform throughout the hybrid sponge. Use of the PLGA sponge as a 
      skeleton facilitated formation of the hybrid sponge into desired shapes with high 
      mechanical strength while collagen microsponges contributed good cell interaction 
      and hydrophilicity.
CI  - Copyright 2000 John Wiley & Sons, Inc.
FAU - Chen, G
AU  - Chen G
AD  - 3D Tissue Engineering Group, National Institute for Advanced Interdisciplinary 
      Research, 1-1-4 Higashi, Tsukuba, Ibaraki, Japan. chen@nair.go.jp
FAU - Ushida, T
AU  - Ushida T
FAU - Tateishi, T
AU  - Tateishi T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biomed Mater Res
JT  - Journal of biomedical materials research
JID - 0112726
RN  - 0 (Biocompatible Materials)
RN  - 0 (Polymers)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Biocompatible Materials/chemistry/*pharmacokinetics
MH  - Biodegradation, Environmental
MH  - *Collagen
MH  - Electron Probe Microanalysis
MH  - L Cells
MH  - Lactic Acid/chemistry/*pharmacokinetics
MH  - Mice
MH  - Microscopy, Electron, Scanning
MH  - Polyglycolic Acid/chemistry/*pharmacokinetics
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Polymers/chemistry/*pharmacokinetics
EDAT- 2000/05/29 09:00
MHDA- 2000/07/25 11:00
CRDT- 2000/05/29 09:00
PHST- 2000/05/29 09:00 [pubmed]
PHST- 2000/07/25 11:00 [medline]
PHST- 2000/05/29 09:00 [entrez]
AID - 10.1002/(SICI)1097-4636(200008)51:2<273::AID-JBM16>3.0.CO;2-O [pii]
AID - 10.1002/(sici)1097-4636(200008)51:2<273::aid-jbm16>3.0.co;2-o [doi]
PST - ppublish
SO  - J Biomed Mater Res. 2000 Aug;51(2):273-9. doi: 
      10.1002/(sici)1097-4636(200008)51:2<273::aid-jbm16>3.0.co;2-o.