PMID- 10825256
OWN - NLM
STAT- MEDLINE
DCOM- 20000808
LR  - 20240109
IS  - 0951-4198 (Print)
IS  - 0951-4198 (Linking)
VI  - 14
IP  - 10
DP  - 2000
TI  - Matrix-assisted laser desorption/ionization time-of-flight mass spectrometric 
      detection of bacterial biomarker proteins isolated from contaminated water, 
      lettuce and cotton cloth.
PG  - 911-7
AB  - Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass 
      spectra of bacterial proteins were obtained from water, lettuce and cloth samples 
      contaminated with Shigella flexneri, Escherichia coli, and Aeromonas hydrophila. 
      Spectra were obtained using proteins directly isolated from water (or water used 
      for rinsing samples) without culturing the bacteria. For S. flexneri and E. coli, 
      two marker ions for specific proteins associated with a virulence-related 
      property (acid resistance) were easily detected. For A. hydrophila, ions from two 
      specifically selected marker proteins, as well as ions from the larger group of 
      proteins isolated from pure cultures, all matched spectra from a contaminated 
      water sample, providing strong evidence that A. hydrophila was the bacterial 
      contaminant. Rinse water from contaminated lettuce and cloth samples showed the 
      same marker ions as the contaminated water samples.
CI  - Copyright 2000 John Wiley & Sons, Ltd.
FAU - Holland, R D
AU  - Holland RD
AD  - National Center for Toxicological Research, Jefferson, AR 72079, USA.
FAU - Rafii, F
AU  - Rafii F
FAU - Heinze, T M
AU  - Heinze TM
FAU - Sutherland, J B
AU  - Sutherland JB
FAU - Voorhees, K J
AU  - Voorhees KJ
FAU - Lay, J O Jr
AU  - Lay JO Jr
LA  - eng
PT  - Journal Article
PL  - England
TA  - Rapid Commun Mass Spectrom
JT  - Rapid communications in mass spectrometry : RCM
JID - 8802365
RN  - 0 (Bacterial Proteins)
RN  - 0 (Biomarkers)
RN  - 0 (Ions)
SB  - IM
MH  - Aeromonas hydrophila/isolation & purification
MH  - Bacterial Proteins/*analysis
MH  - Biomarkers/analysis
MH  - Escherichia coli/isolation & purification
MH  - *Food Microbiology
MH  - Gossypium/*microbiology
MH  - Ions
MH  - Lasers
MH  - Lactuca/*microbiology
MH  - Shigella flexneri/isolation & purification
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods
MH  - *Water Microbiology
EDAT- 2000/05/29 09:00
MHDA- 2000/08/12 11:00
CRDT- 2000/05/29 09:00
PHST- 2000/05/29 09:00 [pubmed]
PHST- 2000/08/12 11:00 [medline]
PHST- 2000/05/29 09:00 [entrez]
AID - 10.1002/(SICI)1097-0231(20000530)14:10<911::AID-RCM965>3.0.CO;2-C [pii]
AID - 10.1002/(SICI)1097-0231(20000530)14:10<911::AID-RCM965>3.0.CO;2-C [doi]
PST - ppublish
SO  - Rapid Commun Mass Spectrom. 2000;14(10):911-7. doi: 
      10.1002/(SICI)1097-0231(20000530)14:10<911::AID-RCM965>3.0.CO;2-C.