PMID- 10827151
OWN - NLM
STAT- MEDLINE
DCOM- 20000621
LR  - 20240513
IS  - 0270-9139 (Print)
IS  - 0270-9139 (Linking)
VI  - 31
IP  - 6
DP  - 2000 Jun
TI  - Unique epithelial cell production of hepatocyte growth factor/scatter factor by 
      putative precancerous intestinal metaplasias and associated "intestinal-type" 
      biliary cancer chemically induced in rat liver.
PG  - 1257-65
AB  - Recently, we observed that Met, the receptor for hepatocyte growth factor/scatter 
      factor (HGF/SF), is overexpressed in epithelial cells of both early-appearing 
      intestinal metaplastic glands in precancerous hepatic cholangiofibrotic tissue 
      and neoplastic glands in later developed intestinal-type of cholangiocarcinoma 
      originated from the furan rat model of cholangiocarcinogenesis when compared with 
      normal and hyperplastic intrahepatic biliary epithelia. We now show that HGF/SF 
      is also aberrantly expressed in a manner closely paralleling that of its receptor 
      in the neoplastic epithelial cells of furan-induced rat cholangiocarcinomas and 
      in a majority of metaplastic epithelial cells within earlier formed precancerous 
      hepatic cholangiofibrotic tissue. Using in situ hybridization and reverse 
      transcription-polymerase chain reaction (RT-PCR), we further showed specific 
      expression of HGF/SF messenger RNA (mRNA) in a novel rat cholangiocarcinoma 
      epithelial cell line overexpressing Met. This cholangiocarcinoma cell line, 
      termed C611B, was established from tumorigenic cells isolated from a 
      furan-induced transplantable tumor. Moreover, we detected by in situ 
      hybridization strong expression of HGF/SF mRNA transcripts in the cancerous 
      epithelial glands of cholangiocarcinoma developed in recipient rats after in vivo 
      cell transplantation of C611B cells. In contrast, mRNA transcripts and protein 
      immunoreactivity for this cytokine were not detected in hepatocytes and biliary 
      epithelial cells in adult normal rat liver nor in rat hyperplastic intrahepatic 
      biliary epithelium. Our results clearly show that HGF/SF becomes aberrantly 
      expressed in cholangiocarcinoma epithelium and in putative precancerous 
      intestinal metaplastic epithelium induced in the liver of furan-treated rats.
FAU - Lai, G H
AU  - Lai GH
AD  - Department of Pathology, The Medical School at Virginia Commonwealth University, 
      Richmond, VA 23298-0297, USA.
FAU - Radaeva, S
AU  - Radaeva S
FAU - Nakamura, T
AU  - Nakamura T
FAU - Sirica, A E
AU  - Sirica AE
LA  - eng
GR  - 1R01 CA83650/CA/NCI NIH HHS/United States
GR  - 5R01 CA39225/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Hepatology
JT  - Hepatology (Baltimore, Md.)
JID - 8302946
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Animals
MH  - Bile Duct Neoplasms/chemically induced/*metabolism/pathology
MH  - *Bile Ducts, Intrahepatic
MH  - Blotting, Northern
MH  - Cholangiocarcinoma/metabolism/pathology
MH  - Epithelial Cells/metabolism
MH  - Hepatocyte Growth Factor/*biosynthesis
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Intestinal Neoplasms/*metabolism
MH  - Intestines/pathology
MH  - Male
MH  - Metaplasia
MH  - Precancerous Conditions/*metabolism
MH  - Rats
MH  - Rats, Inbred F344
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Cells, Cultured
EDAT- 2000/05/29 09:00
MHDA- 2000/06/24 11:00
CRDT- 2000/05/29 09:00
PHST- 2000/05/29 09:00 [pubmed]
PHST- 2000/06/24 11:00 [medline]
PHST- 2000/05/29 09:00 [entrez]
AID - S0270913900988918 [pii]
AID - 10.1053/jhep.2000.8108 [doi]
PST - ppublish
SO  - Hepatology. 2000 Jun;31(6):1257-65. doi: 10.1053/jhep.2000.8108.