PMID- 10827167
OWN - NLM
STAT- MEDLINE
DCOM- 20000914
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 275
IP  - 32
DP  - 2000 Aug 11
TI  - Crystal structure of dTDP-4-keto-6-deoxy-D-hexulose 3,5-epimerase from 
      Methanobacterium thermoautotrophicum complexed with dTDP.
PG  - 24608-12
AB  - Deoxythymidine diphosphate (dTDP)-4-keto-6-deoxy-d-hexulose 3, 5-epimerase (RmlC) 
      is involved in the biosynthesis of dTDP-l-rhamnose, which is an essential 
      component of the bacterial cell wall. The crystal structure of RmlC from 
      Methanobacterium thermoautotrophicum was determined in the presence and absence 
      of dTDP, a substrate analogue. RmlC is a homodimer comprising a central jelly 
      roll motif, which extends in two directions into longer beta-sheets. Binding of 
      dTDP is stabilized by ionic interactions to the phosphate group and by a 
      combination of ionic and hydrophobic interactions with the base. The active site, 
      which is located in the center of the jelly roll, is formed by residues that are 
      conserved in all known RmlC sequence homologues. The conservation of the active 
      site residues suggests that the mechanism of action is also conserved and that 
      the RmlC structure may be useful in guiding the design of antibacterial drugs.
FAU - Christendat, D
AU  - Christendat D
AD  - Division of Molecular and Structural Biology, Ontario Cancer Institute, Toronto, 
      Ontario M5G 2M9, Canada.
FAU - Saridakis, V
AU  - Saridakis V
FAU - Dharamsi, A
AU  - Dharamsi A
FAU - Bochkarev, A
AU  - Bochkarev A
FAU - Pai, E F
AU  - Pai EF
FAU - Arrowsmith, C H
AU  - Arrowsmith CH
FAU - Edwards, A M
AU  - Edwards AM
LA  - eng
SI  - PDB/1EP0
SI  - PDB/1EPZ
GR  - RR07707/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Macromolecular Substances)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Thymine Nucleotides)
RN  - 491-97-4 (thymidine 5'-diphosphate)
RN  - EC 5.1.3.- (Carbohydrate Epimerases)
RN  - EC 5.1.3.13 (dTDP-4-ketorhamnose 3,5-epimerase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Binding Sites
MH  - Carbohydrate Epimerases/*chemistry/genetics/*metabolism
MH  - Crystallography, X-Ray
MH  - Dimerization
MH  - Macromolecular Substances
MH  - Methanobacterium/*enzymology/genetics
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Protein Structure, Secondary
MH  - Recombinant Proteins/chemistry/metabolism
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Thymine Nucleotides/chemistry/*metabolism
EDAT- 2000/05/29 09:00
MHDA- 2000/09/19 11:01
CRDT- 2000/05/29 09:00
PHST- 2000/05/29 09:00 [pubmed]
PHST- 2000/09/19 11:01 [medline]
PHST- 2000/05/29 09:00 [entrez]
AID - S0021-9258(19)62214-3 [pii]
AID - 10.1074/jbc.C000238200 [doi]
PST - ppublish
SO  - J Biol Chem. 2000 Aug 11;275(32):24608-12. doi: 10.1074/jbc.C000238200.