PMID- 10827168
OWN - NLM
STAT- MEDLINE
DCOM- 20000921
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 275
IP  - 33
DP  - 2000 Aug 18
TI  - Association of calcium/calmodulin-dependent kinase II with developmentally 
      regulated splice variants of the postsynaptic density protein densin-180.
PG  - 25061-4
AB  - In a continuing search for proteins that target calcium/calmodulin-dependent 
      protein kinase II (CaMKII) to postsynaptic density (PSD) substrates important in 
      synaptic plasticity, we showed that the PSD protein densin-180 binds CaMKII. Four 
      putative splice variants (A-D) of the cytosolic tail of densin-180 are shown to 
      be differentially expressed during brain development. Densin-180 splicing affects 
      CaMKII phosphorylation of specific serine residues. Variants A, B, and D, but not 
      C, bind CaMKII stoichiometrically and with high affinity, mediated by a 
      differentially spliced domain. Densin-180 differs from the previously identified 
      CaMKII-binding protein NR2B in that binding does not strictly require CaMKII 
      autophosphorylation. Binding of densin-180 and NR2B to CaMKII is noncompetitive, 
      indicating different interaction sites on CaMKII. Expression of the 
      membrane-targeted CaMKII-binding domain of densin-180 confers membrane 
      localization to coexpressed CaMKII without requiring calcium mobilization, 
      suggesting that densin-180 plays a role in the constitutive association of CaMKII 
      with PSDs.
FAU - Strack, S
AU  - Strack S
AD  - Department of Molecular Physiology and Biophysics and Center for Molecular 
      Neuroscience, Vanderbilt University Medical Center, Nashville, TN 37232-0615, 
      USA.
FAU - Robison, A J
AU  - Robison AJ
FAU - Bass, M A
AU  - Bass MA
FAU - Colbran, R J
AU  - Colbran RJ
LA  - eng
SI  - GENBANK/AF266164
GR  - CA68485/CA/NCI NIH HHS/United States
GR  - DK20593/DK/NIDDK NIH HHS/United States
GR  - NS37508/NS/NINDS NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Lrrc7 protein, mouse)
RN  - 0 (Lrrc7 protein, rat)
RN  - 0 (Protein Isoforms)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Sialoglycoproteins)
RN  - EC 2.5.1.18 (Glutathione Transferase)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
SB  - IM
MH  - *Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Binding Sites
MH  - Brain/metabolism
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 2
MH  - Calcium-Calmodulin-Dependent Protein Kinases/*metabolism
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Cytosol/chemistry
MH  - Glutathione Transferase/metabolism
MH  - Kinetics
MH  - Mice
MH  - Microscopy, Fluorescence
MH  - Molecular Sequence Data
MH  - Phosphorylation
MH  - Protein Binding
MH  - Protein Isoforms
MH  - Protein Structure, Tertiary
MH  - Rats
MH  - Recombinant Fusion Proteins/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sialoglycoproteins/chemistry/*metabolism
EDAT- 2000/05/29 09:00
MHDA- 2000/09/23 11:01
CRDT- 2000/05/29 09:00
PHST- 2000/05/29 09:00 [pubmed]
PHST- 2000/09/23 11:01 [medline]
PHST- 2000/05/29 09:00 [entrez]
AID - S0021-9258(19)62056-9 [pii]
AID - 10.1074/jbc.C000319200 [doi]
PST - ppublish
SO  - J Biol Chem. 2000 Aug 18;275(33):25061-4. doi: 10.1074/jbc.C000319200.