PMID- 10827205
OWN - NLM
STAT- MEDLINE
DCOM- 20000623
LR  - 20220409
IS  - 0022-3166 (Print)
IS  - 0022-3166 (Linking)
VI  - 130
IP  - 6
DP  - 2000 Jun
TI  - A high fructose diet affects the early steps of insulin action in muscle and 
      liver of rats.
PG  - 1531-5
AB  - A high fructose diet induces insulin resistance in rats, although the exact 
      molecular mechanism involved is unknown. In this study, we used 
      immunoprecipitation and immunoblotting to examine the levels and phosphorylation 
      status of the insulin receptor (IR) and insulin receptor substrate-1 (IRS-1), as 
      well as the association of the IRS-1 with phosphatidylinositol 3-kinase (PI 
      3-kinase), and phosphotyrosine phosphatase (SHP2) in the liver and muscle of rats 
      fed a control or high fructose diet for 28 d. There were no differences in IR and 
      the IRS-1 protein levels in the liver and muscle of rats fed the control and high 
      fructose diets. However, tyrosine-phosphorylation of the insulin receptor after 
      insulin stimulation was reduced to 71 +/- 2% (P < 0.05) of control in the liver 
      of the fructose-fed rats. In samples previously immunoprecipitated with 
      anti-IRS-1 antibody and blotted with antiphosphotyrosine antibody, the 
      insulin-stimulated IRS-1 phosphorylation levels in the liver and muscle of the 
      fructose-fed group were only 70 +/- 6% (P < 0.05) and 76 +/- 5% (P < 0.05) of 
      those of control rats, respectively. The insulin-stimulated IRS-1 association 
      with PI 3-kinase was reduced to 84 +/- 3% (P < 0.05) in the liver and to 84 +/- 
      4% (P < 0.05) in the muscle of the fructose-fed group compared with control rats. 
      Insulin-stimulated IRS-1 association with SHP2 was reduced to 79 +/- 5% (P < 
      0.05) in liver of the fructose-fed rats. These data suggest that changes in the 
      early steps of insulin signal transduction may have an important role in the 
      insulin resistance observed in these rats.
FAU - Bezerra, R M
AU  - Bezerra RM
AD  - Departamento de Planejamento Alimentar e Nutricao, Faculdade de Engenharia de 
      Alimentos and Departamento de Clinica Medica, Faculdade de Ciencias Medicas, 
      Universidade Estadual de Campinas, Campinas, SP, Brasil.
FAU - Ueno, M
AU  - Ueno M
FAU - Silva, M S
AU  - Silva MS
FAU - Tavares, D Q
AU  - Tavares DQ
FAU - Carvalho, C R
AU  - Carvalho CR
FAU - Saad, M J
AU  - Saad MJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Nutr
JT  - The Journal of nutrition
JID - 0404243
RN  - 0 (Blood Glucose)
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Insulin)
RN  - 0 (Insulin Receptor Substrate Proteins)
RN  - 0 (Irs1 protein, rat)
RN  - 0 (Phosphoproteins)
RN  - 30237-26-4 (Fructose)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.10.1 (Receptor, Insulin)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases)
SB  - IM
MH  - Animals
MH  - Blood Glucose/drug effects
MH  - Body Weight/drug effects
MH  - Dietary Carbohydrates/administration & dosage/*pharmacology
MH  - Fructose/administration & dosage/*pharmacology
MH  - Insulin/*metabolism
MH  - Insulin Receptor Substrate Proteins
MH  - Liver/*drug effects/enzymology/metabolism
MH  - Male
MH  - Muscle, Skeletal/*drug effects/metabolism
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Phosphoproteins/drug effects/metabolism
MH  - Phosphorylation/drug effects
MH  - Protein Tyrosine Phosphatases/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor, Insulin/*drug effects/metabolism
EDAT- 2000/05/29 09:00
MHDA- 2000/07/06 11:00
CRDT- 2000/05/29 09:00
PHST- 2000/05/29 09:00 [pubmed]
PHST- 2000/07/06 11:00 [medline]
PHST- 2000/05/29 09:00 [entrez]
AID - 10.1093/jn/130.6.1531 [doi]
PST - ppublish
SO  - J Nutr. 2000 Jun;130(6):1531-5. doi: 10.1093/jn/130.6.1531.