PMID- 10828031
OWN - NLM
STAT- MEDLINE
DCOM- 20000629
LR  - 20220129
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 95
IP  - 11
DP  - 2000 Jun 1
TI  - Acquisition of intact allogeneic human leukocyte antigen molecules by human 
      dendritic cells.
PG  - 3473-7
AB  - In an attempt to transduce monocyte-derived dendritic cells (DCs) by a retroviral 
      vector coding for a cell surface marker, we were confronted by the observation of 
      high transfer of the surface molecule in the absence of vector proviral DNA in 
      the treated cells. Indeed, DCs acquired the surface marker by a mechanism 
      independent of the vector machinery, requiring cell-to-cell contact and involving 
      transfer of lipids and a variety of intact membrane proteins. Most important, 
      this property of DCs also includes acquisition of foreign human leukocyte antigen 
      (HLA) molecules. Consequently, DCs become immunological hybrids as they display 
      their own and foreign HLA molecules. The newly acquired HLA is fully functional 
      because it allows recognition by allo-specific T lymphocytes and the binding and 
      presentation of antigen peptides.
FAU - Russo, V
AU  - Russo V
AD  - HSR-Telethon Institute of Gene Therapy (TIGET) and the Cancer Immunotherapy and 
      Gene Therapy Program, H.S. Raffaele Scientific Institute, Milan, Italy.
FAU - Zhou, D
AU  - Zhou D
FAU - Sartirana, C
AU  - Sartirana C
FAU - Rovere, P
AU  - Rovere P
FAU - Villa, A
AU  - Villa A
FAU - Rossini, S
AU  - Rossini S
FAU - Traversari, C
AU  - Traversari C
FAU - Bordignon, C
AU  - Bordignon C
LA  - eng
GR  - TGT00F04/TI_/Telethon/Italy
GR  - TGT06S01/TI_/Telethon/Italy
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Isoantigens)
RN  - 0 (Membrane Lipids)
RN  - 0 (Membrane Proteins)
SB  - IM
MH  - Cell Communication/physiology
MH  - Cell Membrane/immunology/physiology
MH  - Dendritic Cells/*immunology
MH  - Flow Cytometry
MH  - HLA-DR Antigens/*metabolism
MH  - Humans
MH  - Isoantigens/*metabolism
MH  - Melanoma
MH  - Membrane Lipids/metabolism
MH  - Membrane Proteins/metabolism
MH  - Monocytes/immunology
MH  - T-Lymphocytes/*immunology
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 2000/05/29 09:00
MHDA- 2000/07/06 11:00
CRDT- 2000/05/29 09:00
PHST- 2000/05/29 09:00 [pubmed]
PHST- 2000/07/06 11:00 [medline]
PHST- 2000/05/29 09:00 [entrez]
AID - S0006-4971(20)64013-7 [pii]
PST - ppublish
SO  - Blood. 2000 Jun 1;95(11):3473-7.