PMID- 10828603
OWN - NLM
STAT- MEDLINE
DCOM- 20000626
LR  - 20171101
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 88
IP  - 3-4
DP  - 2000
TI  - Five human genes encoding F-box proteins: chromosome mapping and analysis in 
      human tumors.
PG  - 255-8
AB  - Members of the F-box protein (Fbp) family are characterized by an approximately 
      40 amino acid F-box motif. SCF complexes (formed by Skp1, cullin, and one of many 
      Fbps) act as protein-ubiquitin ligases that control the G(1)/S transition of the 
      eukaryotic cell cycle. The substrate specificity of SCF complexes is determined 
      by the presence of different Fbp subunits that recruit specific substrates for 
      ubiquitination. Unchecked degradation of cellular regulatory proteins has been 
      observed in certain tumors and it is possible that deregulated ubiquitin ligases 
      play a role in the altered degradation of cell cycle regulators. We have recently 
      identified a family of human Fbps. As a first step aimed at determining if FBP 
      genes could be involved in human neoplasia, we have mapped the chromosome 
      positions of 5 FBP genes by fluorescence in situ hybridization (FISH) to 10q24 
      (BTRC alias beta-TRCP/FBW1a), 9q34 (FBXW2 alias FBW2), 13q22 (FBXL3A alias 
      FBL3a), 5p12 (FBXO4 alias FBX4) and 6q25-->q26 (FBXO5 alias FBX5). Since most of 
      these are chromosomal loci frequently altered in tumors, we have screened 42 
      human tumor cell lines and 48 human tumor samples by Southern hybridization and 
      FISH. While no gross alterations of the genes encoding beta-Trcp/Fbw1a, Fbw2, 
      Fbx4 and Fbx5 were found, heterozygous deletion of the FBXL3A gene was found in 
      four of 13 small cell carcinoma cell lines. This is the first evaluation of genes 
      encoding Fbps in human tumors.
CI  - Copyright 2000 S. Karger AG, Basel
FAU - Chiaur, D S
AU  - Chiaur DS
AD  - Department of Pathology, New York University School of Medicine, New York, NY 
      10016, USA.
FAU - Murthy, S
AU  - Murthy S
FAU - Cenciarelli, C
AU  - Cenciarelli C
FAU - Parks, W
AU  - Parks W
FAU - Loda, M
AU  - Loda M
FAU - Inghirami, G
AU  - Inghirami G
FAU - Demetrick, D
AU  - Demetrick D
FAU - Pagano, M
AU  - Pagano M
LA  - eng
GR  - CA66229/CA/NCI NIH HHS/United States
GR  - CA76584/CA/NCI NIH HHS/United States
GR  - GM57587/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - EC 2.3.2.27 (SKP Cullin F-Box Protein Ligases)
RN  - EC 6.3.2.- (Peptide Synthases)
SB  - IM
MH  - Amino Acid Motifs
MH  - Blotting, Southern
MH  - Carcinoma, Small Cell/genetics
MH  - Chromosome Aberrations/genetics
MH  - Chromosome Deletion
MH  - Female
MH  - Genetic Testing
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lung Neoplasms/genetics
MH  - Male
MH  - Multigene Family/*genetics
MH  - Neoplasms/*genetics
MH  - Peptide Synthases/analysis/*chemistry/*genetics
MH  - *Physical Chromosome Mapping
MH  - SKP Cullin F-Box Protein Ligases
MH  - Tumor Cells, Cultured
EDAT- 2000/06/01 09:00
MHDA- 2000/07/06 11:00
CRDT- 2000/06/01 09:00
PHST- 2000/06/01 09:00 [pubmed]
PHST- 2000/07/06 11:00 [medline]
PHST- 2000/06/01 09:00 [entrez]
AID - 15532 [pii]
AID - 10.1159/000015532 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 2000;88(3-4):255-8. doi: 10.1159/000015532.