PMID- 10828677
OWN - NLM
STAT- MEDLINE
DCOM- 20000829
LR  - 20180213
IS  - 0302-2838 (Print)
IS  - 0302-2838 (Linking)
VI  - 37
IP  - 6
DP  - 2000 Jun
TI  - Effect of beta-sitosterol on transforming growth factor-beta-1 expression and 
      translocation protein kinase C alpha in human prostate stromal cells in vitro.
PG  - 735-41
AB  - BACKGROUND: Today, plant extracts are widely used in the treatment of benign 
      prostatic hyperplasia (BPH). However, the complete mode of action of the active 
      substance, beta-sitosterol, is under investigation. The purpose of this study was 
      to investigate the effect of beta-sitosterol on the expression of transforming 
      growth factor beta 1 (TGF-beta1) and the activity of protein kinase C alpha 
      (PKC-alpha) in primary prostate stromal cell cultures in vitro. METHODS: Tissue 
      samples for primary cell cultures were obtained from patients undergoing 
      transurethral resection of the prostate (TURP). TGF-beta1 levels in stromal cell 
      conditioned media following a culture with beta-sitosterol were detected in a 
      TGF-beta1 specific ELISA assay. Following different incubation periods with 
      beta-sitosterol, cells were lysed and fractionated into a Triton-soluble membrane 
      fraction and a cytosol fraction. PKC-alpha protein was detected using immunoblot 
      analysis. RESULTS: Beta-sitosterol was able to induce the expression and 
      secretion of TGF-beta1 significantly between 1.26- and 1.86-fold compared to a 
      cholesterol and the nonsupplemented control in 6 of 8 individual cultures. The 
      total amount of secreted TGF-beta1 varied in cells from different patients. Based 
      on its presence in both membrane fraction and cytosol, PKC-alpha appeared to be 
      constitutively expressed in stromal cells. In the absence of beta-sitosterol 
      PKC-alpha was predominantly found in its membrane-associated active form. 
      Following a culture with beta-sitosterol, a translocation of PKC-alpha from the 
      membrane to the cytosol was observed. This effect was specific for 
      beta-sitosterol as compared to cholesterol. CONCLUSION: This study describes the 
      effect of beta-sitosterol on the expression of a multifunctional growth factor 
      (TGF-beta1) and the activity of PKC-alpha membrane in stromal cells of the human 
      prostate in vitro.
FAU - Kassen, A
AU  - Kassen A
AD  - Wissenschaftliches Labor der Urologischen Klinik der Ruhr-Universitat Bochum, 
      Deutschland. Annette.Kassen@ruhr-uni-bochum.de
FAU - Berges, R
AU  - Berges R
FAU - Senge, T
AU  - Senge T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Eur Urol
JT  - European urology
JID - 7512719
RN  - 0 (Isoenzymes)
RN  - 0 (Sitosterols)
RN  - 0 (Transforming Growth Factor beta)
RN  - 5LI01C78DD (gamma-sitosterol)
RN  - EC 2.7.11.13 (PRKCA protein, human)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 2.7.11.13 (Protein Kinase C-alpha)
SB  - IM
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Humans
MH  - Isoenzymes/*metabolism
MH  - Male
MH  - Prostate/*cytology/*enzymology
MH  - Protein Kinase C/*metabolism
MH  - Protein Kinase C-alpha
MH  - Sitosterols/*pharmacology
MH  - Time Factors
MH  - Transforming Growth Factor beta/*biosynthesis
EDAT- 2000/06/01 09:00
MHDA- 2000/09/02 11:01
CRDT- 2000/06/01 09:00
PHST- 2000/06/01 09:00 [pubmed]
PHST- 2000/09/02 11:01 [medline]
PHST- 2000/06/01 09:00 [entrez]
AID - 20227 [pii]
AID - 10.1159/000020227 [doi]
PST - ppublish
SO  - Eur Urol. 2000 Jun;37(6):735-41. doi: 10.1159/000020227.