PMID- 10830276
OWN - NLM
STAT- MEDLINE
DCOM- 20000615
LR  - 20071114
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 141
IP  - 6
DP  - 2000 Jun
TI  - Blood to brain transfer of leptin in normal and interleukin-1beta-treated male 
      rats.
PG  - 1951-4
AB  - To test the hypothesis that leptin was secreted from the brain into the blood of 
      the rat, its concentration was measured in the superior sagittal sinus (SSS; 
      which drains the cerebral cortex) and aortic blood of normal fasting male rats 
      and rats that had been treated with iv or intracerebroventricular (icv) 
      injections of interleukin-1beta (IL-1beta; 100 ng), a cytokine previously shown 
      to induce peripheral leptin secretion. Plasma levels of leptin in SSS were 
      slightly, but significantly, less than those in the aorta in control, 
      saline-injected rats (0.99+/-0.07 vs. 1.19+/-0.10 ng/ml; n = 15; P = 0.03) and in 
      rats injected with human IL-1beta iv (1.56+/-0.12 vs. 1.92+/-0.15 ng/ml; n = 23; 
      P = 0.004) or icv (1.38+/-0.11 vs. 1.57+/-0.12 ng/ml; n = 23; P = 0.008). 
      IL-1beta by either the iv or icv route significantly increased leptin levels in 
      the aorta [1.19+/-0.10 vs. 1.92+/-0.15 ng/ml (P = 0.0002) and 1.19+/-0.10 vs. 
      1.57+/-0.12 ng/ml (P = 0.022), respectively]. SSS levels of leptin were also 
      raised after iv or icv injection (P = 0.0002 and P = 0.0053, respectively). These 
      findings demonstrate a net uptake of leptin by the cerebral cortex from 
      peripheral blood in both normal and IL-1beta-treated animals and show that 
      peripheral blood levels of leptin are increased by IL-1beta whether administered 
      icv or iv.
FAU - Reichlin, S
AU  - Reichlin S
AD  - Department of Medicine, University of Arizona College of Medicine, Tucson 
      85724-5099, USA. reichlin@arizona.edu
FAU - Chen, G
AU  - Chen G
FAU - Nicolson, M
AU  - Nicolson M
LA  - eng
GR  - 16894/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Interleukin-1)
RN  - 0 (Leptin)
SB  - IM
MH  - Animals
MH  - Aorta
MH  - *Blood-Brain Barrier
MH  - Cerebral Cortex/blood supply
MH  - Humans
MH  - Injections, Intravenous
MH  - Injections, Intraventricular
MH  - Interleukin-1/administration & dosage/*pharmacology
MH  - Leptin/*metabolism
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2000/06/01 09:00
MHDA- 2000/06/17 09:00
CRDT- 2000/06/01 09:00
PHST- 2000/06/01 09:00 [pubmed]
PHST- 2000/06/17 09:00 [medline]
PHST- 2000/06/01 09:00 [entrez]
AID - 10.1210/endo.141.6.7459 [doi]
PST - ppublish
SO  - Endocrinology. 2000 Jun;141(6):1951-4. doi: 10.1210/endo.141.6.7459.