PMID- 10831409
OWN - NLM
STAT- MEDLINE
DCOM- 20000818
LR  - 20210526
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 66
IP  - 6
DP  - 2000 Jun
TI  - Isolation and expression of lactate dehydrogenase genes from Rhizopus oryzae.
PG  - 2343-8
AB  - Rhizopus oryzae is used for industrial production of lactic acid, yet little is 
      known about the genetics of this fungus. In this study I cloned two genes, ldhA 
      and ldhB, which code for NAD(+)-dependent L-lactate dehydrogenases (LDH) (EC 
      1.1.1.27), from a lactic acid-producing strain of R. oryzae. These genes are 
      similar to each other and exhibit more than 90% nucleotide sequence identity and 
      they contain no introns. This is the first description of ldh genes in a fungus, 
      and sequence comparisons revealed that these genes are distinct from previously 
      isolated prokaryotic and eukaryotic ldh genes. Protein sequencing of the LDH 
      isolated from R. oryzae during lactic acid production confirmed that ldhA codes 
      for a 36-kDa protein that converts pyruvate to lactate. Production of LdhA was 
      greatest when glucose was the carbon source, followed by xylose and trehalose; 
      all of these sugars could be fermented to lactic acid. Transcripts from ldhB were 
      not detected when R. oryzae was grown on any of these sugars but were present 
      when R. oryzae was grown on glycerol, ethanol, and lactate. I hypothesize that 
      ldhB encodes a second NAD(+)-dependent LDH that is capable of converting 
      L-lactate to pyruvate and is produced by cultures grown on these nonfermentable 
      substrates. Both ldhA and ldhB restored fermentative growth to Escherichia coli 
      (ldhA pfl) mutants so that they grew anaerobically and produced lactic acid.
FAU - Skory, C D
AU  - Skory CD
AD  - Fermentation Biochemistry Research, National Center for Agricultural Utilization 
      Research, Agricultural Research Service, U.S. Department of Agriculture, Peoria, 
      Illinois 61604-3902, USA. skorycd@mail.usda.gov
LA  - eng
SI  - GENBANK/AF226154
SI  - GENBANK/AF226155
PT  - Journal Article
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Culture Media)
RN  - 0U46U6E8UK (NAD)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
SB  - IM
MH  - Animals
MH  - Blotting, Southern
MH  - Cloning, Molecular
MH  - Culture Media
MH  - Escherichia coli/enzymology/genetics
MH  - Gene Expression Regulation, Fungal
MH  - Genes, Fungal
MH  - Genetic Complementation Test
MH  - Humans
MH  - L-Lactate Dehydrogenase/*genetics/*metabolism
MH  - Molecular Sequence Data
MH  - NAD/metabolism
MH  - Polymerase Chain Reaction
MH  - Rhizopus/enzymology/*genetics/growth & development
MH  - Sequence Analysis, DNA
MH  - Transcription, Genetic
PMC - PMC110528
EDAT- 2000/06/01 09:00
MHDA- 2000/08/29 11:01
PMCR- 2000/06/01
CRDT- 2000/06/01 09:00
PHST- 2000/06/01 09:00 [pubmed]
PHST- 2000/08/29 11:01 [medline]
PHST- 2000/06/01 09:00 [entrez]
PHST- 2000/06/01 00:00 [pmc-release]
AID - 1116 [pii]
AID - 10.1128/AEM.66.6.2343-2348.2000 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2000 Jun;66(6):2343-8. doi: 
      10.1128/AEM.66.6.2343-2348.2000.