PMID- 10833301
OWN - NLM
STAT- MEDLINE
DCOM- 20000727
LR  - 20151119
IS  - 1044-7431 (Print)
IS  - 1044-7431 (Linking)
VI  - 15
IP  - 5
DP  - 2000 May
TI  - Sequential activation of the 5-HT1(A) serotonin receptor and TrkB induces the 
      serotonergic neuronal phenotype.
PG  - 446-55
AB  - Serotonin (5-HT) is an important factor controlling survival, differentiation, 
      and plasticity of neurons in serotonergic target regions of the brain and has 
      been implicated in major psychiatric and autonomic disorders. Relatively little 
      is known, however, of factors controlling differentiation and plasticity of 
      developing and adult 5-HT neurons. We show now that 5-HT, the 5-HT1(A) receptor, 
      brain-derived neurotrophic factor (BDNF), and its receptor, trkB, form an 
      auto/paracrine loop for the regulation of the serotonergic phenotype. Serotonin 
      applied to cultures from E14 rat raphe increased numbers of neurons expressing 
      serotonergic markers in a dose-dependent manner. Agonists of the 5-HT1(A) 
      receptor, BP-554 and 8-OH-DPAT, but not agonists of the 5-HT1(B) and 5-HT1(D) 
      receptors, mimicked this effect, while the specific 5-HT1(A) antagonist, 
      WAY-100635, inhibited it. Serotonin also increased BDNF mRNA and protein in 
      embryonic raphe cultures. Induction of serotonergic markers by serotonin was 
      suppressed by a trkB-IgG fusion protein but not by trkC-IgG. Taken together, our 
      data indicate that serotonin acts on 5-HT1(A) autoreceptors, causing 
      up-regulation of BDNF, which activates trkB to promote serotonergic 
      phenotype-specific markers.
CI  - Copyright 2000 Academic Press.
FAU - Galter, D
AU  - Galter D
AD  - Department of Neuroanatomy, University of Heidelberg, Germany.
FAU - Unsicker, K
AU  - Unsicker K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cell Neurosci
JT  - Molecular and cellular neurosciences
JID - 9100095
RN  - 0 (Biomarkers)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Carbazoles)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Indole Alkaloids)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Serotonin)
RN  - 0 (Receptors, Serotonin, 5-HT1)
RN  - 0 (Serotonin Receptor Agonists)
RN  - 333DO1RDJY (Serotonin)
RN  - 97161-97-2 (staurosporine aglycone)
RN  - EC 2.7.- (Phosphotransferases)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.10.1 (Receptor, trkC)
SB  - IM
MH  - Animals
MH  - Biomarkers
MH  - Brain-Derived Neurotrophic Factor/genetics
MH  - Carbazoles/pharmacology
MH  - Cells, Cultured
MH  - Enzyme Inhibitors/pharmacology
MH  - Indole Alkaloids
MH  - Neurons/*physiology
MH  - Phenotype
MH  - Phosphotransferases/antagonists & inhibitors
MH  - RNA, Messenger/metabolism
MH  - Raphe Nuclei/cytology/drug effects/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor, trkB/*physiology
MH  - Receptor, trkC/physiology
MH  - Receptors, Serotonin/*physiology
MH  - Receptors, Serotonin, 5-HT1
MH  - Serotonin/pharmacology/*physiology
MH  - Serotonin Receptor Agonists/pharmacology
EDAT- 2000/06/02 00:00
MHDA- 2000/06/02 00:01
CRDT- 2000/06/02 00:00
PHST- 2000/06/02 00:00 [pubmed]
PHST- 2000/06/02 00:01 [medline]
PHST- 2000/06/02 00:00 [entrez]
AID - S1044-7431(00)90841-8 [pii]
AID - 10.1006/mcne.2000.0841 [doi]
PST - ppublish
SO  - Mol Cell Neurosci. 2000 May;15(5):446-55. doi: 10.1006/mcne.2000.0841.