PMID- 10836421
OWN - NLM
STAT- MEDLINE
DCOM- 20000719
LR  - 20190915
IS  - 0969-8043 (Print)
IS  - 0969-8043 (Linking)
VI  - 52
IP  - 5
DP  - 2000 May
TI  - Novel data set for retrospective biodosimetry using both conventional and FISH 
      chromosome analysis after high accidental overexposure.
PG  - 1149-52
AB  - Blood samples of ten Chernobyl and one non-Chernobyl victims were analysed both 
      by conventional cytogenetics and by fluorescence in situ hybridization (FISH) 
      using a cocktail of chromosomes 2, 3 and 8. The analysed group comprised men 
      acutely irradiated mainly in 1986 and aged 26-47 years at the time of first blood 
      sampling. All of them displayed acute radiation syndrome of varying severity. 
      Chromosome analysis of the earliest blood samples was carried out by conventional 
      scoring of unstable aberrations with the number of metaphases analysed per 
      individual ranging from 35 to 300. Estimated individual doses ranged from 0.85 to 
      9.8 G y. After a 10 year delay, i.e. in 1996, blood samples were analysed both by 
      conventional scoring of unstable aberrations and by FISH measurements of stable 
      ones. Usually about 500 metaphases per individual were scored. Estimated by the 
      FISH-method individual translocation (tc + ti) frequencies ranged from 2.2 to 
      116.8 per 100 cells full genome equivalent. Based on three different published 
      dicentric dose response, in vitro curves individual doses were calculated from 
      the earliest dicentric frequencies. A dose response curve for truly persisting 
      translocations (tc + ti) was estimated over the range 1-10 Gy.
FAU - Sevan'kaev, A V
AU  - Sevan'kaev AV
AD  - Medical Radiological Research Centre, Obninsk, Russia.
FAU - Khvostunov, I K
AU  - Khvostunov IK
FAU - Mikhailova, G F
AU  - Mikhailova GF
FAU - Golub, E V
AU  - Golub EV
FAU - Potetnya, O I
AU  - Potetnya OI
FAU - Shepel, N N
AU  - Shepel NN
FAU - Nugis, V Y
AU  - Nugis VY
FAU - Nadejina, N M
AU  - Nadejina NM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Appl Radiat Isot
JT  - Applied radiation and isotopes : including data, instrumentation and methods for 
      use in agriculture, industry and medicine
JID - 9306253
SB  - IM
MH  - Adult
MH  - *Chromosome Aberrations
MH  - Dose-Response Relationship, Radiation
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Lymphocytes/radiation effects/ultrastructure
MH  - Male
MH  - Middle Aged
MH  - *Radioactive Hazard Release
MH  - Radiometry/*methods
MH  - Retrospective Studies
EDAT- 2000/06/03 09:00
MHDA- 2000/07/25 11:00
CRDT- 2000/06/03 09:00
PHST- 2000/06/03 09:00 [pubmed]
PHST- 2000/07/25 11:00 [medline]
PHST- 2000/06/03 09:00 [entrez]
AID - S0969804300000622 [pii]
AID - 10.1016/s0969-8043(00)00062-2 [doi]
PST - ppublish
SO  - Appl Radiat Isot. 2000 May;52(5):1149-52. doi: 10.1016/s0969-8043(00)00062-2.