PMID- 10836544
OWN - NLM
STAT- MEDLINE
DCOM- 20001017
LR  - 20190921
IS  - 1011-1344 (Print)
IS  - 1011-1344 (Linking)
VI  - 54
IP  - 2-3
DP  - 2000 Feb
TI  - Preferential cytotoxicity for multidrug-resistant K562 cells using the 
      combination of a photosensitizer and a cyanine dye.
PG  - 136-44
AB  - The cyanine dye 1,1',3,3,3',3'-hexamethylindodicarbocyanine iodide (HIDC) 
      protects K562 leukemia cells from photodynamic membrane damage caused by 
      cis-di(4-sulfonatophenyl)diphenylporphine (TPPS2) and 420 nm light. This 
      wavelength of light is chosen because it is absorbed by TPPS2, but not by HIDC. 
      The photodynamic system studied may be useful as a model for antineoplastic 
      therapy. A subline of K562 leukemia (K562/DOX), expressing the 
      multidrug-resistance (MDR) phenotype, is found to accumulate smaller amounts of 
      HIDC than the parent cell line and thus has less photoprotection. In the absence 
      of added HIDC, the K562/DOX cell line is more resistant to photodynamic 
      cytotoxicity than the K562 cell line. The resistance of the K562/DOX cell line is 
      not due to a smaller accumulation of TPPS2 than the K562 cell line. However, when 
      both cell lines are incubated with HIDC and TPPS2, and then exposed to light, the 
      K562/DOX cell line becomes more sensitive to photodynamic cell damage than the 
      K562 cell line. The combination of a photosensitizer with a cationic or 
      lysomorphotropic photoprotector represents a novel strategy for the eradication 
      of malignant cells expressing the MDR phenotype.
FAU - Kanofsky, J R
AU  - Kanofsky JR
AD  - Medical Service, Edward Hines, Jr., Department of Veterans Affairs Hospital, 
      Hines, IL 60641, USA. jeff.kanofsky@med.va.gov
FAU - Sima, P D
AU  - Sima PD
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Switzerland
TA  - J Photochem Photobiol B
JT  - Journal of photochemistry and photobiology. B, Biology
JID - 8804966
RN  - 0 (Coloring Agents)
RN  - 0 (Cyanides)
RN  - 0 (Photosensitizing Agents)
RN  - 0 (Porphyrins)
RN  - 35218-75-8 (tetraphenylporphine sulfonate)
SB  - IM
MH  - Cell Membrane/drug effects/pathology
MH  - Coloring Agents/*pharmacology
MH  - Cyanides/metabolism/*pharmacology
MH  - Drug Resistance
MH  - Humans
MH  - K562 Cells
MH  - Photosensitizing Agents/*pharmacology
MH  - Porphyrins/*pharmacology
EDAT- 2000/06/03 09:00
MHDA- 2000/10/21 11:01
CRDT- 2000/06/03 09:00
PHST- 2000/06/03 09:00 [pubmed]
PHST- 2000/10/21 11:01 [medline]
PHST- 2000/06/03 09:00 [entrez]
AID - S1011-1344(00)00008-7 [pii]
AID - 10.1016/s1011-1344(00)00008-7 [doi]
PST - ppublish
SO  - J Photochem Photobiol B. 2000 Feb;54(2-3):136-44. doi: 
      10.1016/s1011-1344(00)00008-7.