PMID- 10837140
OWN - NLM
STAT- MEDLINE
DCOM- 20000816
LR  - 20031114
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 257
IP  - 2
DP  - 2000 Jun 15
TI  - The nucleoside diphosphate kinase activity of DRnm23 is not required for 
      inhibition of differentiation and induction of apoptosis in 32Dcl3 myeloid 
      precursor cells.
PG  - 265-71
AB  - DRnm23 belongs to a multigene family which includes nm23-H1, the first bona fide 
      metastasis suppressor gene, nm23-H2, nm23-H4, and nm23-H5. Like nm23-H1, nm23-H2, 
      and nm23-H4, DRnm23 possesses nucleoside diphosphate kinase (NDPK) activity. Upon 
      overexpression in myeloid precursor 32Dcl3 cells, DRnm23 inhibits granulocytic 
      differentiation and promotes apoptosis. Two specific mutants of DRnm23 (H134Q and 
      S136P), at residues required for the NDPK activity, inhibit differentiation and 
      promote apoptosis of 32Dcl3 cells. By contrast, substitution of serine 61 with 
      proline (S61P) or deletion of the RGD domain (DeltaRGD) abrogates the effects of 
      wild-type DRnm23. Like wild-type DRnm23, all four mutants show a predominantly 
      mitochondrial subcellular localization. These studies indicate that the enzymatic 
      activity of DRnm23 is not required for the effects observed in 32Dcl3 cells. 
      Moreover, the inability of the S61P and DeltaRGD DRnm23 mutants to inhibit 
      differentiation and promote apoptosis may be due to defective protein-protein 
      interactions at the mitochondria, the predominant site of DRnm23 subcellular 
      localization.
CI  - Copyright 2000 Academic Press.
FAU - Venturelli, D
AU  - Venturelli D
AD  - Department of Microbiology and Immunology, Thomas Jefferson University, 
      Philadelphia, Pennsylvania, 19107, USA.
FAU - Cesi, V
AU  - Cesi V
FAU - Ransac, S
AU  - Ransac S
FAU - Engelhard, A
AU  - Engelhard A
FAU - Perrotti, D
AU  - Perrotti D
FAU - Calabretta, B
AU  - Calabretta B
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - EC 2.7.4.6 (Nucleoside-Diphosphate Kinase)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Cell Differentiation
MH  - Cell Line
MH  - Cell Survival
MH  - Gene Expression
MH  - Granulocytes/*cytology
MH  - Hematopoietic Stem Cells/*cytology/enzymology
MH  - Mice
MH  - Mutagenesis
MH  - Nucleoside-Diphosphate Kinase/genetics/*metabolism
MH  - Subcellular Fractions
MH  - Transfection
EDAT- 2000/06/06 09:00
MHDA- 2000/08/19 11:00
CRDT- 2000/06/06 09:00
PHST- 2000/06/06 09:00 [pubmed]
PHST- 2000/08/19 11:00 [medline]
PHST- 2000/06/06 09:00 [entrez]
AID - S0014-4827(00)94899-7 [pii]
AID - 10.1006/excr.2000.4899 [doi]
PST - ppublish
SO  - Exp Cell Res. 2000 Jun 15;257(2):265-71. doi: 10.1006/excr.2000.4899.