PMID- 10837367
OWN - NLM
STAT- MEDLINE
DCOM- 20000713
LR  - 20171116
IS  - 1044-1549 (Print)
IS  - 1044-1549 (Linking)
VI  - 22
IP  - 6
DP  - 2000 Jun
TI  - Modification of type I collagenous gels by alveolar epithelial cells.
PG  - 702-7
AB  - Contraction of type I collagen gels is an in vitro model of tissue remodeling. In 
      addition to fibroblasts, some epithelial cells can mediate this process. We 
      therefore hypothesized that alveolar epithelial cells might contract 
      extracellular matrices and have the potential to directly participate in the 
      remodeling of the lung after alveolar injury. A549 cells were plated on top of 
      collagen gels, and the gels were floated in culture medium. A549 cells contracted 
      the gels in a time- and cell density-dependent manner. A549 cells, as well as 
      human bronchial epithelial cells (HBEC) and rat alveolar epithelial cells 
      (RalvEC) contracted collagen gels more when they were plated on top of the gel 
      than when they were embedded inside, in contrast to human fetal lung fibroblast 
      (HFL1), which contracted more when cast inside. The amount of hydroxyproline in 
      the collagen gels remained unchanged throughout the contraction. Anti-beta(1) 
      integrin antibody inhibited A549 cell-mediated contraction. Transforming growth 
      factor beta augmented the contraction by A549 cells as well as that by HBEC and 
      HFL1. Prostaglandin E(2) inhibited the contraction by HFL1 but did not affect the 
      contraction by A549 cells, HBEC, or RalvEC. Cytomix (a mixture of tumor necrosis 
      factor-alpha, interleukin-1beta, and interferon-gamma) inhibited the contraction 
      by HFL1 but strongly enhanced the contraction by A549 cells. Cytomix also caused 
      a morphologic change of A549 cells from a polygonal to a spindle shape. 
      Immunocytochemistry showed that cytomix induced alpha-tubulin expression in A549 
      cells, whereas cytokeratin, vimentin, smooth muscle actin, beta(1) integrin, and 
      paxillin expressions were not changed. This study thus demonstrates that alveolar 
      epithelial cells can cause contraction of extracellular matrices and that this 
      process is modulated by exogenous mediators, which also modify the microtubular 
      system. Such an activity might contribute to alveolar remodeling after injury.
FAU - Umino, T
AU  - Umino T
AD  - Department of Internal Medicine, University of Nebraska Medical Center, Omaha, 
      Nebraska 68198-5125, USA.
FAU - Wang, H
AU  - Wang H
FAU - Zhu, Y
AU  - Zhu Y
FAU - Liu, X
AU  - Liu X
FAU - Manouilova, L S
AU  - Manouilova LS
FAU - Spurzem, J R
AU  - Spurzem JR
FAU - Patricia Leuschen, M
AU  - Patricia Leuschen M
FAU - Rennard, S I
AU  - Rennard SI
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Respir Cell Mol Biol
JT  - American journal of respiratory cell and molecular biology
JID - 8917225
RN  - 0 (Antibodies)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Gels)
RN  - 0 (Integrin beta1)
RN  - 0 (PXN protein, human)
RN  - 0 (Paxillin)
RN  - 0 (Phosphoproteins)
RN  - 0 (Pxn protein, rat)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Tubulin)
RN  - 9007-34-5 (Collagen)
RN  - K7Q1JQR04M (Dinoprostone)
RN  - RMB44WO89X (Hydroxyproline)
SB  - IM
MH  - Animals
MH  - Antibodies/pharmacology
MH  - Bronchi/*cytology
MH  - Cell Count/drug effects
MH  - Cell Culture Techniques/methods
MH  - Cell Size/physiology
MH  - Collagen/*metabolism/*pharmacology
MH  - Cytoskeletal Proteins/analysis
MH  - Dinoprostone/pharmacology
MH  - Epithelial Cells/chemistry/drug effects/*metabolism
MH  - Fetus/cytology
MH  - Fluorescent Antibody Technique
MH  - Gels
MH  - Humans
MH  - Hydroxyproline/analysis
MH  - Integrin beta1/immunology
MH  - Paxillin
MH  - Phenotype
MH  - Phosphoproteins/analysis
MH  - Pulmonary Alveoli/*cytology/physiology
MH  - Rats
MH  - Transforming Growth Factor beta/pharmacology
MH  - Tubulin/analysis
MH  - Tumor Cells, Cultured
EDAT- 2000/06/06 09:00
MHDA- 2000/07/15 11:00
CRDT- 2000/06/06 09:00
PHST- 2000/06/06 09:00 [pubmed]
PHST- 2000/07/15 11:00 [medline]
PHST- 2000/06/06 09:00 [entrez]
AID - 10.1165/ajrcmb.22.6.3806 [doi]
PST - ppublish
SO  - Am J Respir Cell Mol Biol. 2000 Jun;22(6):702-7. doi: 10.1165/ajrcmb.22.6.3806.