PMID- 10841361
OWN - NLM
STAT- MEDLINE
DCOM- 20000906
LR  - 20220316
IS  - 0959-4965 (Print)
IS  - 0959-4965 (Linking)
VI  - 11
IP  - 7
DP  - 2000 May 15
TI  - GFP fluorescence reports Period 1 circadian gene regulation in the mammalian 
      biological clock.
PG  - 1479-82
AB  - Endogenous cyclic activation of a specific set of genes, including Period 1 
      (Per1), drive circadian rhythms in the suprachiasmatic nucleus (SCN), a 
      biological clock nucleus of the brain. We have produced transgenic mice in which 
      a degradable form of recombinant jellyfish green fluorescent protein (GFP) is 
      driven by the mouse Period 1 (mPer1) gene promoter. GFP protein is expressed in 
      the circadian neural structures of the retina and SCN. Fluorescent signals are 
      resolved at the level of individual neurons. mPer1-driven GFP fluorescence 
      intensity reports light-induction and circadian rhythmicity in SCN neurons. This 
      circadian reporter transgene captures the gene expression dynamics of living 
      biological clock neurons and ensembles, providing a novel view of this brain 
      function.
FAU - Kuhlman, S J
AU  - Kuhlman SJ
AD  - Department of Physiology, University of Kentucky, Lexington 40536-0084, USA.
FAU - Quintero, J E
AU  - Quintero JE
FAU - McMahon, D G
AU  - McMahon DG
LA  - eng
GR  - AG13426/AG/NIA NIH HHS/United States
GR  - MH60794/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Neuroreport
JT  - Neuroreport
JID - 9100935
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Per1 protein, mouse)
RN  - 0 (Period Circadian Proteins)
RN  - 0 (RNA, Messenger)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Biological Clocks/*physiology
MH  - Cell Cycle Proteins
MH  - Female
MH  - Gene Expression Regulation/physiology
MH  - Genes, Reporter
MH  - Green Fluorescent Proteins
MH  - In Situ Hybridization
MH  - Indicators and Reagents/*metabolism
MH  - Luminescent Proteins/*genetics
MH  - Male
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Mice, Transgenic
MH  - Nuclear Proteins/*genetics
MH  - Period Circadian Proteins
MH  - RNA, Messenger/analysis
MH  - Suprachiasmatic Nucleus/*physiology
MH  - Transgenes/physiology
EDAT- 2000/06/07 09:00
MHDA- 2000/09/09 11:01
CRDT- 2000/06/07 09:00
PHST- 2000/06/07 09:00 [pubmed]
PHST- 2000/09/09 11:01 [medline]
PHST- 2000/06/07 09:00 [entrez]
PST - ppublish
SO  - Neuroreport. 2000 May 15;11(7):1479-82.