PMID- 10842241
OWN - NLM
STAT- MEDLINE
DCOM- 20000724
LR  - 20191104
IS  - 0022-3034 (Print)
IS  - 0022-3034 (Linking)
VI  - 43
IP  - 3
DP  - 2000 Jun 5
TI  - Differential effects of the trophic factors BDNF, NT-4, GDNF, and IGF-I on the 
      isthmo-optic nucleus in chick embryos.
PG  - 289-303
AB  - The isthmo-optic nucleus (ION) of chick embryos is a model system for the study 
      of retrograde trophic signaling in developing CNS neurons. The role of 
      brain-derived neurotrophic factor (BDNF) is well established in this system. 
      Recent work has implicated neurotrophin-4 (NT-4), glial cell line-derived 
      neurotrophic factor (GDNF), and insulin-like growth factor I (IGF-I) as 
      additional trophic factors for ION neurons. Here it was examined in vitro and in 
      vivo whether these factors are target-derived trophic factors for the ION in 13- 
      to 16-day-old chick embryos. Unlike BDNF, neither GDNF, NT-4, nor IGF-I increased 
      the survival of ION neurons in dissociated cultures identified by retrograde 
      labeling with the fluorescent tracer DiI. BDNF and IGF-I promoted neurite 
      outgrowth from ION explants, whereas GDNF and NT-4 had no effect. Injections of 
      NT-4, but not GDNF, in the retina decreased the survival of ION neurons and 
      accelerated cell death in the ION. NT-4-like immunoreactivity was present in the 
      retina and the ION. Exogenous, radiolabeled NT-4, but not GDNF or IGF-I, was 
      retrogradely transported from the retina to the ION. NT-4 transport was 
      significantly reduced by coinjection of excess cold nerve growth factor (NGF), 
      indicating that the majority of NT-4 bound to p75 neurotrophin receptors during 
      axonal transport. Binding of NT-4 to chick p75 receptors was confirmed in 
      L-cells, which express chick p75 receptors. These data indicate that GDNF has no 
      direct trophic effects on ION neurons. IGF-I may be an afferent trophic factor 
      for the ION, and NT-4 may act as an antagonist to BDNF, either by competing with 
      BDNF for p75 and/or trkB binding or by signaling cell death via p75.
CI  - Copyright 2000 John Wiley & Sons, Inc.
FAU - Janiga, T A
AU  - Janiga TA
AD  - Department of Physiology and Cell Biology, University of Nevada School of 
      Medicine, Reno, Nevada 89557, USA.
FAU - Rind, H B
AU  - Rind HB
FAU - von Bartheld, C S
AU  - von Bartheld CS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurobiol
JT  - Journal of neurobiology
JID - 0213640
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Carrier Proteins)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - P658DCA9XD (neurotrophin 4)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects/physiology
MH  - Axonal Transport/drug effects/physiology
MH  - Brain-Derived Neurotrophic Factor/*metabolism/*pharmacology
MH  - Carrier Proteins/drug effects/metabolism
MH  - Cell Count
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Chick Embryo
MH  - Glial Cell Line-Derived Neurotrophic Factor
MH  - Immunoblotting
MH  - Immunohistochemistry
MH  - Insulin-Like Growth Factor I/*metabolism/*pharmacology
MH  - Mesencephalon/*drug effects/embryology/*metabolism
MH  - Nerve Growth Factors/*metabolism/*pharmacology
MH  - Nerve Tissue Proteins/drug effects/*metabolism/*pharmacology
MH  - Neurites/drug effects/metabolism/ultrastructure
MH  - Receptors, Nerve Growth Factor/drug effects/metabolism
MH  - Retina/drug effects/embryology/metabolism
MH  - Superior Colliculi/cytology/drug effects/metabolism
MH  - Visual Pathways/*drug effects/embryology/*metabolism
EDAT- 2000/06/08 09:00
MHDA- 2000/08/01 11:00
CRDT- 2000/06/08 09:00
PHST- 2000/06/08 09:00 [pubmed]
PHST- 2000/08/01 11:00 [medline]
PHST- 2000/06/08 09:00 [entrez]
AID - 10.1002/(SICI)1097-4695(20000605)43:3<289::AID-NEU7>3.0.CO;2-5 [pii]
AID - 10.1002/(sici)1097-4695(20000605)43:3<289::aid-neu7>3.0.co;2-5 [doi]
PST - ppublish
SO  - J Neurobiol. 2000 Jun 5;43(3):289-303. doi: 
      10.1002/(sici)1097-4695(20000605)43:3<289::aid-neu7>3.0.co;2-5.