PMID- 10843737
OWN - NLM
STAT- MEDLINE
DCOM- 20000823
LR  - 20071115
IS  - 1043-4666 (Print)
IS  - 1043-4666 (Linking)
VI  - 12
IP  - 6
DP  - 2000 Jun
TI  - Expression and regulation of histidine decarboxylase mRNA expression in the 
      uterus during pregnancy in the mouse.
PG  - 622-9
AB  - It has been hypothesized that hormonally regulated histamine production plays a 
      role in preparation of the uterus for implantation. Histidine decarboxylase (HDC) 
      is the rate-limiting enzyme for histamine production. The current study was 
      designed to determine intrauterine expression of HDC mRNA expression during 
      pregnancy in the mouse. High levels of HDC mRNA expression were observed in the 
      preimplantation mouse uterus with peak expression occurring on day 4. High levels 
      of HDC mRNA expression were also detected in the post-implantation uterus. In an 
      effort to determine whether HDC mRNA is regulated by pro-inflammatory cytokines, 
      the HDC mRNA pattern was compared to intrauterine expression of mRNA's for 
      interleukin-1alpha (IL-1alpha), IL-1beta, macrophage chemotactic protein-1 
      (MCP-1) and RANTES (regulated on activation, normal T expressed and secreted) 
      during the peri-implantation period. IL-1beta, MCP-1 and RANTES mRNA levels were 
      increased in the uterus on days 1-2 and on days 4-5. Increased expression of 
      IL-1alpha mRNA was observed on days 1-2 and days 5-7. There was no clear 
      relationship between HDC mRNA expression and cytokine/chemokine mRNA expression. 
      Progesterone-stimulated intrauterine expression of HDC mRNA. Intrauterine 
      cytokine/chemokine mRNA was also hormonally regulated. This data allowed the 
      possibility that one or more of these pro-inflammatory cytokines could be 
      involved in regulating intrauterine HDC mRNA production. Recombinant IL-1alpha, 
      IL-1beta, MCP-1 and RANTES all failed to induce HDC mRNA expression in the 
      preimplantation uterus in a mouse pseudopregnancy model. At the same time, 
      IL-1beta induced the expression of mRNA for each of the four 
      cytokines/chemokines. Despite the fact that these were also produced in the 
      uterus during pregnancy and were hormonally regulated, none of these cytokines 
      induced intrauterine HDC mRNA expression. The data suggest that progesterone is 
      involved in the regulation of HDC mRNA expression in the preimplantation uterus, 
      but IL-1alpha/beta, MCP-1 and RANTES, which have been reported to regulate 
      histamine synthesis during inflammatory processes, do not appear to play a role.
CI  - Copyright 2000 Academic Press.
FAU - Wood, G W
AU  - Wood GW
AD  - Department of Pathology and Laboratory Medicine, University of Kansas Medical 
      Center, Kansas City, Kansas, USA. woodg@karmanos.org
FAU - Hausmann, E H
AU  - Hausmann EH
FAU - Choudhuri, R
AU  - Choudhuri R
FAU - Dileepan, K N
AU  - Dileepan KN
LA  - eng
GR  - HD30802/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1)
RN  - 0 (RNA, Messenger)
RN  - EC 4.1.1.22 (Histidine Decarboxylase)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/genetics
MH  - Chemokine CCL5/genetics
MH  - Cytokines/*genetics
MH  - Embryo Implantation
MH  - Female
MH  - *Gene Expression Regulation
MH  - Gene Expression Regulation, Enzymologic
MH  - Histidine Decarboxylase/*genetics
MH  - Interleukin-1/genetics
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Pregnancy
MH  - Pregnancy, Animal/immunology/*physiology
MH  - RNA, Messenger/genetics
MH  - T-Lymphocytes/immunology
MH  - *Transcription, Genetic
MH  - Uterus/*enzymology/*immunology
EDAT- 2000/06/14 09:00
MHDA- 2000/08/29 11:01
CRDT- 2000/06/14 09:00
PHST- 2000/06/14 09:00 [pubmed]
PHST- 2000/08/29 11:01 [medline]
PHST- 2000/06/14 09:00 [entrez]
AID - S1043-4666(00)90667-7 [pii]
AID - 10.1006/cyto.2000.0667 [doi]
PST - ppublish
SO  - Cytokine. 2000 Jun;12(6):622-9. doi: 10.1006/cyto.2000.0667.