PMID- 10844593
OWN - NLM
STAT- MEDLINE
DCOM- 20000824
LR  - 20131121
IS  - 0085-2538 (Print)
IS  - 0085-2538 (Linking)
VI  - 57
IP  - 6
DP  - 2000 Jun
TI  - Cox-2 and osteopontin in cocultured platelets and mesangial cells: role of 
      glucocorticoids.
PG  - 2229-38
AB  - BACKGROUND: Glomerular inflammation is characterized by a consecutive 
      infiltration of immunoreactive cells. To mimic the early phase of glomerular 
      injury, a coculture system of platelets and rat renal mesangial cells was 
      established. As prototypes, the inflammation-related proteins cyclooxygenase-2 
      (Cox-2) and the chemotactic protein osteopontin (OPN) were investigated. METHODS: 
      The expression of OPN and Cox-2 mRNA and protein was determined by Northern and 
      Western blot analyses. RESULTS: Coincubation of platelets and mesangial cells led 
      to a rapid, transient induction of Cox-2 mRNA, which peaked at two hours, whereas 
      OPN and monocyte chemoattractant protein-1 (MCP-1) were induced at later time 
      points. The induction of Cox-2 mRNA was concentration dependent and highly 
      reproducible when platelets of different donors were investigated. Partial Cox-2 
      induction was observed when supernatants of preactivated platelets were incubated 
      with mesangial cells. The inhibition of the signaling pathways of 
      platelet-derived growth factor (PDGF) and epidermal growth factor (EGF) or 
      interference with Gi-protein signaling partially inhibited platelet-induced Cox-2 
      expression. Down-regulation of protein kinase C (PKC), which is a common 
      signaling module in many pathways leading to Cox-2 induction, almost completely 
      abrogated platelet-induced Cox-2 expression. The time pattern of Cox-2 and OPN 
      expression suggested that Cox-2 might play a role in OPN induction. The 
      up-regulation of OPN was dependent on de novo protein synthesis and was induced 
      by high levels of exogenous prostaglandin E2 (PGE2; 10 micromol/L). Endogenous 
      PGE2, however, proved not to be essential for OPN mRNA expression, because 
      inhibition of Cox activity did not change OPN mRNA levels. Dexamethasone 
      inhibited Cox-2 mRNA induction but increased OPN mRNA and protein expression. 
      CONCLUSION: These data indicate that Cox-2 and OPN are independently up-regulated 
      upon interaction of platelets and mesangial cells.
FAU - Goppelt-Struebe, M
AU  - Goppelt-Struebe M
AD  - Medizinische Klinik IV, Universitat Erlangen-Nurnberg, Erlangen, and Institut fur 
      Anatomie II, Albert-Ludwigs Universitat Freiburg, Freiburg, Germany. 
      Goppelt-Struebe@rzmail.uni-erlangen.de
FAU - Wiedemann, T
AU  - Wiedemann T
FAU - Heusinger-Ribeiro, J
AU  - Heusinger-Ribeiro J
FAU - Vucadinovic, M
AU  - Vucadinovic M
FAU - Rehm, M
AU  - Rehm M
FAU - Prols, F
AU  - Prols F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Kidney Int
JT  - Kidney international
JID - 0323470
RN  - 0 (Glucocorticoids)
RN  - 0 (Growth Substances)
RN  - 0 (Isoenzymes)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (SPP1 protein, human)
RN  - 0 (Sialoglycoproteins)
RN  - 0 (Spp1 protein, rat)
RN  - 106441-73-0 (Osteopontin)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - EC 1.14.99.1 (PTGS2 protein, human)
RN  - EC 1.14.99.1 (Prostaglandin-Endoperoxide Synthases)
SB  - IM
MH  - Animals
MH  - Blood Platelets/drug effects/*metabolism/physiology
MH  - Cell Division/physiology
MH  - Coculture Techniques
MH  - Cyclooxygenase 2
MH  - Dexamethasone/pharmacology
MH  - Gene Expression Regulation/physiology
MH  - Glomerular Mesangium/cytology/*enzymology/physiology
MH  - Glucocorticoids/pharmacology/*physiology
MH  - Growth Substances/physiology
MH  - Humans
MH  - Isoenzymes/*blood/genetics
MH  - Membrane Proteins
MH  - Osteopontin
MH  - Prostaglandin-Endoperoxide Synthases/*blood/genetics
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Sialoglycoproteins/*blood/genetics
MH  - Up-Regulation
EDAT- 2000/06/09 09:00
MHDA- 2000/08/29 11:01
CRDT- 2000/06/09 09:00
PHST- 2000/06/09 09:00 [pubmed]
PHST- 2000/08/29 11:01 [medline]
PHST- 2000/06/09 09:00 [entrez]
AID - S0085-2538(15)46983-2 [pii]
AID - 10.1046/j.1523-1755.2000.00083.x [doi]
PST - ppublish
SO  - Kidney Int. 2000 Jun;57(6):2229-38. doi: 10.1046/j.1523-1755.2000.00083.x.