PMID- 10845774
OWN - NLM
STAT- MEDLINE
DCOM- 20000802
LR  - 20151119
IS  - 1044-7431 (Print)
IS  - 1044-7431 (Linking)
VI  - 15
IP  - 4
DP  - 2000 Apr
TI  - N-acetyllactosamine and the CT carbohydrate antigen mediate agrin-dependent 
      activation of MuSK and acetylcholine receptor clustering in skeletal muscle.
PG  - 380-97
AB  - Galbeta1,3GalNAc and Galbeta1,4GIcNAc are the subterminal saccharide structures 
      present on the CT carbohydrate antigen 
      GalNAcbeta1,4[NeuAcalpha2,3]-Galbeta1-(3GalNAc or 4GIcNAc)-R, which is localized 
      at the mammalian neuromuscular junction. Here we show that Galbeta1,3GalNAc, 
      Galbeta1,4GIcNAc, and the CT carbohydrate antigen affect postsynaptic assembly in 
      cultured muscle cells. Treatment of C2C12 myotubes with benzyl-O-alpha-GalNAc or 
      neuraminidase increased peanut agglutinin (PNA) expression and AChR clustering. 
      Induction of AChR clustering was blocked by PNA and by muscle agrin. Addition of 
      Galbeta1,4GIcNAc or Galbeta1,3GalNAc increased AChR clustering in myotubes and 
      muscle-specific kinase (MUSK) autophosphorylation in vitro, while 
      NeuAcalpha2,3Galbeta1,4GIcNAc and Galbeta1,4GIc did not. Neural agrin activated 
      MuSK in vitro if the lactosamine-containing mucin domain was present, and this 
      activation was blocked in large part by Galbeta1,3GalNAc and Galbeta1,4GIcNAc. 
      Agrin fragments and MuSK bound to these disaccharides with differing 
      specificities. Overexpression of the CT carbohydrate antigen also increased AChR 
      clustering and MuSK autophosphorylation in the presence of neural agrin. These 
      data suggest a model in which different portions of the CT carbohydrate structure 
      contribute to agrin-dependent signal transduction.
FAU - Parkhomovskiy, N
AU  - Parkhomovskiy N
AD  - Department of Neurosciences, University of California at San Diego School of 
      Medicine, La Jolla 92093-0691, USA.
FAU - Kammesheidt, A
AU  - Kammesheidt A
FAU - Martin, P T
AU  - Martin PT
LA  - eng
GR  - NS37214/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Neurosci
JT  - Molecular and cellular neurosciences
JID - 9100095
RN  - 0 (Agrin)
RN  - 0 (Amino Sugars)
RN  - 0 (Antigens, Tumor-Associated, Carbohydrate)
RN  - 0 (Benzyl Compounds)
RN  - 0 (DNA, Complementary)
RN  - 0 (Gangliosides)
RN  - 0 (Oligopeptides)
RN  - 0 (Peanut Agglutinin)
RN  - 0 (Peptides)
RN  - 0 (Receptors, Cholinergic)
RN  - 122932-83-6 (GalNAc-3'-isoL(M1) ganglioside)
RN  - 13000-25-4 (lactosamine)
RN  - 3554-90-3 (Thomsen-Friedenreich antigen)
RN  - 3554-93-6 (benzyl-alpha-N-acetylgalactosamine)
RN  - 3Y5B2K5OOK (N-acetyllactosamine)
RN  - 98849-88-8 (FLAG peptide)
RN  - EC 2.7.10.1 (MUSK protein, human)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 3.2.1.18 (Neuraminidase)
RN  - KM15WK8O5T (Acetylgalactosamine)
SB  - IM
MH  - Acetylgalactosamine/analogs & derivatives/pharmacology
MH  - Agrin/analysis/genetics/*metabolism
MH  - Amino Sugars/chemistry/*metabolism
MH  - Animals
MH  - Antigens, Tumor-Associated, Carbohydrate/chemistry/metabolism
MH  - Benzyl Compounds/pharmacology
MH  - COS Cells
MH  - Cell Line
MH  - DNA, Complementary
MH  - Extracellular Matrix/drug effects/metabolism
MH  - Gangliosides/chemistry/genetics/*metabolism
MH  - Gene Expression/physiology
MH  - Humans
MH  - Kidney/cytology
MH  - Muscle, Skeletal/chemistry/cytology/*enzymology
MH  - Neuraminidase/pharmacology
MH  - Oligopeptides/genetics
MH  - Peanut Agglutinin
MH  - Peptides/genetics
MH  - Protein Binding/drug effects/physiology
MH  - Protein Structure, Tertiary
MH  - Receptor Protein-Tyrosine Kinases/analysis/genetics/*metabolism
MH  - Receptors, Cholinergic/*metabolism
MH  - Transfection
EDAT- 2000/06/14 09:00
MHDA- 2000/08/06 11:00
CRDT- 2000/06/14 09:00
PHST- 2000/06/14 09:00 [pubmed]
PHST- 2000/08/06 11:00 [medline]
PHST- 2000/06/14 09:00 [entrez]
AID - S1044-7431(00)90835-2 [pii]
AID - 10.1006/mcne.2000.0835 [doi]
PST - ppublish
SO  - Mol Cell Neurosci. 2000 Apr;15(4):380-97. doi: 10.1006/mcne.2000.0835.