PMID- 10846238
OWN - NLM
STAT- MEDLINE
DCOM- 20000720
LR  - 20171116
IS  - 0003-3898 (Print)
IS  - 0003-3898 (Linking)
VI  - 58
IP  - 3
DP  - 2000 May-Jun
TI  - [Use of standard for quantitation of adhesion of polynuclear neutrophils by flow 
      cytometry].
PG  - 337-43
AB  - Adherence receptors are essential for heterotypic (endothelial cell, platelet) 
      polymorphonuclear neutrophil (PMN) interaction. Determination of their expression 
      level give information about activation state and functionality of PMN. Use of 
      flow cytometry associated with an immunolabeling standard, represented by beads 
      coated by a determined amount of immunoglobulins (Qifikit, Dako), allows analysis 
      of specific antibody binding capacity and gives information about antigen 
      density. Using this methodology, the exploration of surface adherence receptors, 
      L-selectin (CD62L) and b2-integrins (CD11a-c/CD18) from PMN unstimulated and 
      incubated with pro-inflammatory stimuli, formyl-methionyl-leucyl-phenylalanine 
      (fMLP) and tumor necrosis factor a (TNFa), allows, on the one hand, the 
      establishment of basal expression values on resting PNN and on the other hand, 
      the study of PNN reactivity. This method of quantification can be applied to 
      clinical studies as adherence receptor deficiency syndromes or inflammatory, 
      infectious and vascular diseases.
FAU - Latger-Cannard, V
AU  - Latger-Cannard V
AD  - Laboratoire d'hematologie, CHU Nancy, 54500 VandOEuvre-les-Nancy.
FAU - Regnault, V
AU  - Regnault V
FAU - Dumas, D
AU  - Dumas D
FAU - Virion, J M
AU  - Virion JM
FAU - Schooneman, J F
AU  - Schooneman JF
FAU - Stoltz, J F
AU  - Stoltz JF
FAU - Lecompte, T
AU  - Lecompte T
LA  - fre
PT  - Journal Article
TT  - Utilisation d'un calibrateur pour quantifier les recepteurs d'adherence du 
      polynucleaire neutrophile par cytometrie en flux.
PL  - France
TA  - Ann Biol Clin (Paris)
JT  - Annales de biologie clinique
JID - 2984690R
RN  - 0 (CD11 Antigens)
RN  - 0 (CD18 Antigens)
RN  - 0 (Reagent Kits, Diagnostic)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 126880-86-2 (L-Selectin)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
SB  - IM
MH  - Adult
MH  - CD11 Antigens/blood
MH  - CD18 Antigens/blood
MH  - Calibration
MH  - *Cell Adhesion/drug effects
MH  - Female
MH  - Flow Cytometry/methods
MH  - Humans
MH  - L-Selectin/blood
MH  - Male
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/drug effects/*physiology
MH  - Reagent Kits, Diagnostic
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 2000/06/10 09:00
MHDA- 2000/07/25 11:00
CRDT- 2000/06/10 09:00
PHST- 2000/06/10 09:00 [pubmed]
PHST- 2000/07/25 11:00 [medline]
PHST- 2000/06/10 09:00 [entrez]
PST - ppublish
SO  - Ann Biol Clin (Paris). 2000 May-Jun;58(3):337-43.