PMID- 10848917
OWN - NLM
STAT- MEDLINE
DCOM- 20000810
LR  - 20190831
IS  - 0954-7894 (Print)
IS  - 0954-7894 (Linking)
VI  - 30
IP  - 7
DP  - 2000 Jul
TI  - Lack of linkage between chromosome 5q23-33 markers and IgE/bronchial 
      hyperreactivity in 67 Scottish families.
PG  - 954-61
AB  - BACKGROUND: Raised serum immunoglobulin E (IgE) and bronchial hyperreactivity 
      (BHR) are risk factors for the expression of the asthma phenotype. Previous 
      studies have reported evidence for linkage between these traits and markers on 
      the 5q23-33 cytokine gene cluster. OBJECTIVE: To test for linkage between total 
      serum IgE/BHR and microsatellite markers which map to the 5q23-33 region in an 
      ethnically distinct cohort of families from Aberdeen, Scotland. METHODS: We 
      performed a linkage study between five polymorphic markers (spanning the 
      chromosome 5q23-33 region) and total serum IgE and BHR traits. A cohort of 67 
      families, who were recruited originally to study the natural history of wheeze, 
      were clinically characterized and genotyped for D5S404, IL4, IRF-1, IL9, D5S436 
      markers. Linkage analyses were performed using the nonparametric Haseman-Elston 
      algorithm for the quantitative trait log IgE, and the nonparametric LOD score 
      (NPL-score) of the GENEHUNTER package for the qualitative traits serum IgE and 
      BHR. RESULTS: The results of the nonparametric linkage analysis using either the 
      Haseman-Elston algorithm or NPL-score were consistent and showed no evidence for 
      linkage with IgE. There was also no evidence for linkage between the BHR traits 
      (at cut-off values of PD20FEV1 < 8 mmol and 16 mmol) and any of the tested five 
      microsatellite markers. CONCLUSIONS: This study presents evidence against the 
      presence of a gene with a major effect on total serum IgE or BHR in the 5q23-33 
      region, in this ethnic group.
FAU - Mansur, A H
AU  - Mansur AH
AD  - Molecular Medicine Unit, Clinical Sciences Building, St. James's University 
      Hospital, Leeds, LS9 7TF, UK.
FAU - Christie, G
AU  - Christie G
FAU - Turner, A
AU  - Turner A
FAU - Bishop, D T
AU  - Bishop DT
FAU - Markham, A F
AU  - Markham AF
FAU - Helms, P
AU  - Helms P
FAU - Morrison, J F
AU  - Morrison JF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Allergy
JT  - Clinical and experimental allergy : journal of the British Society for Allergy 
      and Clinical Immunology
JID - 8906443
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Satellite)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Genetic Markers)
RN  - 0 (IRF1 protein, human)
RN  - 0 (Interferon Regulatory Factor-1)
RN  - 0 (Interleukin-9)
RN  - 0 (Phosphoproteins)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adolescent
MH  - Bronchial Hyperreactivity/*genetics/immunology
MH  - Child
MH  - Chromosomes, Human, Pair 5/*genetics
MH  - DNA Primers/chemistry
MH  - DNA, Satellite/analysis
MH  - DNA-Binding Proteins/genetics
MH  - *Genetic Linkage
MH  - Genetic Markers
MH  - Genotype
MH  - Humans
MH  - Immunoglobulin E/*blood
MH  - Interferon Regulatory Factor-1
MH  - Interferon-gamma/genetics
MH  - Interleukin-4/genetics
MH  - Interleukin-9/genetics
MH  - Lod Score
MH  - Pedigree
MH  - Phosphoproteins/genetics
MH  - Polymerase Chain Reaction
MH  - Scotland
EDAT- 2000/06/10 09:00
MHDA- 2000/08/12 11:00
CRDT- 2000/06/10 09:00
PHST- 2000/06/10 09:00 [pubmed]
PHST- 2000/08/12 11:00 [medline]
PHST- 2000/06/10 09:00 [entrez]
AID - cea855 [pii]
AID - 10.1046/j.1365-2222.2000.00855.x [doi]
PST - ppublish
SO  - Clin Exp Allergy. 2000 Jul;30(7):954-61. doi: 10.1046/j.1365-2222.2000.00855.x.