PMID- 10852918
OWN - NLM
STAT- MEDLINE
DCOM- 20001003
LR  - 20210219
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 275
IP  - 35
DP  - 2000 Sep 1
TI  - Activation of Ca2+/calmodulin-dependent protein kinase II within post-synaptic 
      dendritic spines of cultured hippocampal neurons.
PG  - 27165-71
AB  - To understand the cell signaling of protein kinases, it is essential to monitor 
      their activity in each of the subcellular compartments. Here we developed a 
      method to visualize the activities of Ca(2+)/calmodulin-dependent protein kinase 
      II (CaMKII) in the cytoplasm, plasma membrane, and nucleus, separately, by 
      utilizing targeted phosphorylation motifs and phosphorylation-specific 
      antibodies. This approach was used to monitor the activities of post-synaptic 
      CaMKII in cultured hippocampal neurons. Strong stimulation of the neurons by 
      N-methyl-d-aspartate led to global activations of CaMKII in the cell bodies and 
      dendrites. On the other hand, weak stimulation by removal of Mg(2+) block of 
      N-methyl-d-aspartate receptors induced CaMKII signaling localized within single 
      dendritic spines. Post-synaptic CaMKII is thought to modify synaptic efficiency. 
      The present data for the first time demonstrate the activation of CaMKII 
      localized within single dendritic spines and are consistent with the notion that 
      synaptic efficiency is modified by CaMKII in single or multiple spine level 
      depending on the strength of receptor activation.
FAU - Inagaki, N
AU  - Inagaki N
AD  - Laboratory of Biochemistry, Aichi Cancer Center Research Institute, Chikusa-ku, 
      Nagoya 464-8681, Japan. ninagaki@bs.aistnara.ac.jp
FAU - Nishizawa, M
AU  - Nishizawa M
FAU - Arimura, N
AU  - Arimura N
FAU - Yamamoto, H
AU  - Yamamoto H
FAU - Takeuchi, Y
AU  - Takeuchi Y
FAU - Miyamoto, E
AU  - Miyamoto E
FAU - Kaibuchi, K
AU  - Kaibuchi K
FAU - Inagaki, M
AU  - Inagaki M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA Primers)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 2
MH  - Calcium-Calmodulin-Dependent Protein Kinases/*metabolism
MH  - Cells, Cultured
MH  - DNA Primers
MH  - Dendrites/*enzymology
MH  - Enzyme Activation
MH  - Hippocampus/cytology/*enzymology
MH  - Molecular Sequence Data
MH  - Organelles/enzymology
MH  - Rats
MH  - Synapses/*enzymology
EDAT- 2000/06/15 09:00
MHDA- 2000/10/07 11:01
CRDT- 2000/06/15 09:00
PHST- 2000/06/15 09:00 [pubmed]
PHST- 2000/10/07 11:01 [medline]
PHST- 2000/06/15 09:00 [entrez]
AID - S0021-9258(19)61493-6 [pii]
AID - 10.1074/jbc.M003751200 [doi]
PST - ppublish
SO  - J Biol Chem. 2000 Sep 1;275(35):27165-71. doi: 10.1074/jbc.M003751200.