PMID- 10853837
OWN - NLM
STAT- MEDLINE
DCOM- 20001010
LR  - 20180608
IS  - 0895-3988 (Print)
IS  - 0895-3988 (Linking)
VI  - 13
IP  - 1
DP  - 2000 Mar
TI  - Effect of TCDD on maternal toxicity and chorionic gonadotropin--bioactivity in 
      the immediate post-implantation period of macaque.
PG  - 26-31
AB  - The purpose of this experiment was to observe the alterations in bioactivity of 
      chorionic gonadotropin (CG) associated with early fetal loss (EFL), induced by 
      the environmental toxin TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin) in the 
      cynomolgus macaque. Ten of twelve females administered single doses of 1, 2 or 4 
      microg/kg TCDD on gestational day (GD) 12 had EFL from ten to twenty days later. 
      Seven control animals treated only with the vehicle had normal pregnancies. Blood 
      samples were repeatedly collected for hormone evaluation, from two days before 
      treatment to thirty-one days following treatment. Immunoreactive monkey chorionic 
      gonadotropin (mCG) was measured in serum using ELISA, and bioactive mCG was 
      measured using a luminescence LH/CG bioassay. No change in immunoreactive mCG 
      levels was detected as a result of TCDD, treatment, but bioactive mCG levels were 
      significantly lower in TCDD-treated animals compared to controls. This change in 
      bioactivity of mCG was also reflected in the ratio of mCG bioactivity to mCG 
      immunoreactivity (B/I ratio) which began to rise in normal pregnancies by GD 20, 
      but did not rise in TCDD treated animals. These results demonstrate that normal 
      pregnancy in the monkey, as in humans, is characterized by a post-implantation 
      change in the B/I ratio of CG. These findings therefore suggest that changes in 
      the production of bioactive CG may be used as a biomarker of environmental 
      toxicant exposures which lead to EFL.
FAU - Guo, Y M
AU  - Guo YM
AD  - Institute of Applied Toxicology, Nanjing Medical University, China.
FAU - Wang, S Y
AU  - Wang SY
FAU - Wang, X R
AU  - Wang XR
FAU - Lasley, B
AU  - Lasley B
LA  - eng
PT  - Journal Article
PL  - China
TA  - Biomed Environ Sci
JT  - Biomedical and environmental sciences : BES
JID - 8909524
RN  - 0 (Biomarkers)
RN  - 0 (Chorionic Gonadotropin)
RN  - 0 (Polychlorinated Dibenzodioxins)
RN  - 0 (Teratogens)
SB  - IM
MH  - Animals
MH  - Biological Availability
MH  - Biomarkers/analysis
MH  - Chorionic Gonadotropin/*analysis/pharmacokinetics
MH  - Female
MH  - Fetal Death/*chemically induced/veterinary
MH  - Immunoassay
MH  - Macaca fascicularis
MH  - Maternal Exposure
MH  - Polychlorinated Dibenzodioxins/*toxicity
MH  - Pregnancy
MH  - Teratogens/*toxicity
EDAT- 2000/06/15 09:00
MHDA- 2000/10/14 11:01
CRDT- 2000/06/15 09:00
PHST- 2000/06/15 09:00 [pubmed]
PHST- 2000/10/14 11:01 [medline]
PHST- 2000/06/15 09:00 [entrez]
PST - ppublish
SO  - Biomed Environ Sci. 2000 Mar;13(1):26-31.