PMID- 10856798
OWN - NLM
STAT- MEDLINE
DCOM- 20000824
LR  - 20190728
IS  - 0264-410X (Print)
IS  - 0264-410X (Linking)
VI  - 18
IP  - 27
DP  - 2000 Jul 15
TI  - Analysis of latency in cattle after inoculation with a temperature sensitive 
      mutant of bovine herpesvirus 1 (RLB106).
PG  - 3185-95
AB  - Calves were inoculated with the bovine herpes virus 1 (BHV-1) vaccine strain (RLB 
      106), which is a temperature sensitive mutant. The route of inoculation was 
      intranasal instillation or intramuscular (i.m.) injection (flank or neck). As a 
      control, five calves were given placebo by i.m. injection of the neck. Regardless 
      of the infection route, clinical symptoms did not occur. However, BHV-1 
      neutralizing antibodies were detected after inoculation demonstrating that 
      sero-conversion occurred. At 60 days post-inoculation, dexamethasone was given by 
      i.m. injection to attempt reactivation of RLB 106. Only those calves inoculated 
      by the intranasal route shed virus leading to an increase in BHV-1 specific 
      antibodies. As expected, viral DNA and the latency related-RNA were detected in 
      trigeminal ganglia (TG) of calves inoculated by the intranasal route. In 
      contrast, viral nucleic acid was not detected in TG of calves inoculated by the 
      i.m. route or in calves inoculated with placebo. In cervical ganglia or sacral 
      dorsal root ganglia, viral nucleic acid was not consistently detected. This study 
      provides evidence that efficient latency and reactivation does not occur 
      following i.m. inoculation. Since serum-neutralizing antibodies were detected in 
      all inoculated calves, i.m. inoculation led to sero-conversion.
FAU - Jones, C
AU  - Jones C
AD  - Department of Veterinary and Biomedical Science Center for Biotechnology, 
      University of Nebraska, 68583-0905, Lincoln, NE, USA. cj@unlinfo.unl.edu
FAU - Newby, T J
AU  - Newby TJ
FAU - Holt, T
AU  - Holt T
FAU - Doster, A
AU  - Doster A
FAU - Stone, M
AU  - Stone M
FAU - Ciacci-Zanella, J
AU  - Ciacci-Zanella J
FAU - Webster, C J
AU  - Webster CJ
FAU - Jackwood, M W
AU  - Jackwood MW
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (DNA, Viral)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Administration, Intranasal
MH  - Animals
MH  - Cattle
MH  - DNA, Viral/analysis
MH  - Herpesviridae Infections/*virology
MH  - Herpesvirus 1, Bovine/*isolation & purification
MH  - Injections, Intramuscular
MH  - Mutation
MH  - Nervous System/virology
MH  - RNA, Viral/analysis
MH  - Temperature
MH  - *Virus Latency
EDAT- 2000/06/17 09:00
MHDA- 2000/08/29 11:01
CRDT- 2000/06/17 09:00
PHST- 2000/06/17 09:00 [pubmed]
PHST- 2000/08/29 11:01 [medline]
PHST- 2000/06/17 09:00 [entrez]
AID - S0264410X00001067 [pii]
AID - 10.1016/s0264-410x(00)00106-7 [doi]
PST - ppublish
SO  - Vaccine. 2000 Jul 15;18(27):3185-95. doi: 10.1016/s0264-410x(00)00106-7.