PMID- 10856895
OWN - NLM
STAT- MEDLINE
DCOM- 20000817
LR  - 20190709
IS  - 0022-0795 (Print)
IS  - 0022-0795 (Linking)
VI  - 166
IP  - 1
DP  - 2000 Jul
TI  - Diazepam-binding inhibitor/acyl-CoA-binding protein mRNA and peripheral 
      benzodiazepine receptor mRNA in endocrine and immune tissues after prenatal 
      diazepam exposure of male and female rats.
PG  - 163-71
AB  - Peripheral benzodiazepine (BDZ) receptor (PBR) and diazepam-binding 
      inhibitor/acyl-CoA-binding protein (DBI/ACBP) characterized as a ligand at 
      central BDZ receptors, at PBR with involvement in the regulation of 
      steroidogenesis, and as an intracellular acyl-CoA transporter, are both known to 
      interact with BDZ in adult systems. We investigated their expression after 
      prenatal exposure to BDZ. Diazepam (1.25 mg/kg per day s.c.) was administered to 
      time-pregnant Long Evans rats from gestational day (GD) 14 to 20. Expression of 
      mRNAs encoding for PBR and for DBI/ACBP was studied in the same animals with 
      (33)P-labeled 60 mer oligonucleotides (oligos) by in situ hybridization at GD20, 
      and with (32)P-labeled oligos by Northern blot in steroidogenic and immune organs 
      at postnatal day (PN) 14 and in adult offspring. Prenatal diazepam increased 
      DBI/ACBP mRNA expression in male fetal adrenal and in fetal and PN14 testis. 
      Thymus exhibited increased DBI/ACBP mRNA in male fetuses and in adult female 
      offspring, and reduced organ weight at PN14 in both sexes. In female spleen, an 
      increase in DBI/ACBP mRNA and a decrease in PBR mRNA was seen at PN14. Apart from 
      the finding in spleen, no drug-induced changes in PBR mRNA were observed. The 
      effects of prenatal diazepam were superimposed on treatment-independent sex 
      differences in DBI/ACBP mRNA and PBR mRNA expression. Our data indicate that 
      expression of DBI/ACBP mRNA in steroidogenic and immune organs can be affected by 
      exposure to BDZ during ontogeny, while PBR mRNA expression appears to be less 
      sensitive. They further reveal marked sex differences in the developmental 
      patterns of the two proteins during pre- and postpubertal ontogeny.
FAU - Burgi, B
AU  - Burgi B
AD  - Institute of Pharmacology and Toxicology, University of Zurich, CH-8057 Zurich, 
      Switzerland.
FAU - Lichtensteiger, W
AU  - Lichtensteiger W
FAU - Schlumpf, M
AU  - Schlumpf M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Endocrinol
JT  - The Journal of endocrinology
JID - 0375363
RN  - 0 (Carrier Proteins)
RN  - 0 (Diazepam Binding Inhibitor)
RN  - 0 (Hypnotics and Sedatives)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, GABA-A)
RN  - Q3JTX2Q7TU (Diazepam)
SB  - IM
MH  - Adrenal Glands/embryology/metabolism
MH  - Animals
MH  - Animals, Newborn
MH  - Blotting, Northern
MH  - Carrier Proteins/*genetics
MH  - Diazepam/*toxicity
MH  - Diazepam Binding Inhibitor
MH  - Female
MH  - Hypnotics and Sedatives/*toxicity
MH  - In Situ Hybridization
MH  - Male
MH  - Organ Size/drug effects
MH  - Pregnancy
MH  - *Prenatal Exposure Delayed Effects
MH  - RNA, Messenger/*analysis
MH  - Rats
MH  - Rats, Long-Evans
MH  - Receptors, GABA-A/*genetics
MH  - Spleen/metabolism
MH  - Testis/embryology/metabolism
MH  - Thymus Gland/embryology/metabolism
EDAT- 2000/06/17 09:00
MHDA- 2000/08/19 11:00
CRDT- 2000/06/17 09:00
PHST- 2000/06/17 09:00 [pubmed]
PHST- 2000/08/19 11:00 [medline]
PHST- 2000/06/17 09:00 [entrez]
AID - JOE03613 [pii]
AID - 10.1677/joe.0.1660163 [doi]
PST - ppublish
SO  - J Endocrinol. 2000 Jul;166(1):163-71. doi: 10.1677/joe.0.1660163.