PMID- 10856969
OWN - NLM
STAT- MEDLINE
DCOM- 20000718
LR  - 20190915
IS  - 0033-7587 (Print)
IS  - 0033-7587 (Linking)
VI  - 154
IP  - 1
DP  - 2000 Jul
TI  - The kinetics of postirradiation chromatin restitution as revealed by chromosome 
      aberrations detected by premature chromosome condensation and fluorescence in 
      situ hybridization.
PG  - 87-93
AB  - In a study of X-ray-induced chromosome aberrations in human G(0) lymphocytes 
      irradiated with 4 Gy using premature chromosome condensation (PCC) and 
      fluorescence in situ hybridization (FISH), the time-dependent pattern of 
      chromosome fragments and interchromosomal exchanges involving chromosome 4 was 
      recorded after postirradiation incubation times varying from 0.5 to 46.5 h. 
      Unattached acentric fragments and incomplete interchromosomal exchanges have high 
      initial yields, followed by an exponential decrease, while complete 
      interchromosomal exchanges have almost zero initial yield with a subsequent 
      increase in their number. Plateau values of all yields are reached after about 25 
      h. This temporal variation of aberration yields can consistently be explained by 
      the competition of disruptive PCC stress with the progress of postirradiation 
      structural restitution at the sites of radiation-induced chromatin instabilities. 
      Details of the temporal pattern of incomplete exchanges reflect the different 
      kinetics of the alpha and beta components of the yield of aberrations. The 
      observed large difference between late-PCC and metaphase yields of unattached 
      acentric fragments and the almost perfect conversion from incomplete prematurely 
      condensed chromosomes into complete metaphase exchanges are explained by a 
      difference in the magnitude of chromosome condensation stress between PCC and 
      mitotic conditions. Chromatin sites prone to fragmentation and incompleteness 
      under conditions of PCC can therefore persist as genetic instabilities hidden 
      during mitosis.
FAU - Greinert, R
AU  - Greinert R
AD  - Institute of Medical Physics and Biophysics, University of Gottingen, Germany.
FAU - Detzler, E
AU  - Detzler E
FAU - Harder, D
AU  - Harder D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Radiat Res
JT  - Radiation research
JID - 0401245
RN  - 0 (Chromatin)
SB  - IM
MH  - Chromatin/physiology/*radiation effects
MH  - Chromosome Aberrations
MH  - Chromosomes, Human, Pair 4/*radiation effects
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Kinetics
MH  - Lymphocytes/physiology/*radiation effects
MH  - Resting Phase, Cell Cycle/genetics
EDAT- 2000/06/17 09:00
MHDA- 2000/07/25 11:00
CRDT- 2000/06/17 09:00
PHST- 2000/06/17 09:00 [pubmed]
PHST- 2000/07/25 11:00 [medline]
PHST- 2000/06/17 09:00 [entrez]
AID - 10.1667/0033-7587(2000)154[0087:tkopcr]2.0.co;2 [doi]
PST - ppublish
SO  - Radiat Res. 2000 Jul;154(1):87-93. doi: 
      10.1667/0033-7587(2000)154[0087:tkopcr]2.0.co;2.