PMID- 1085699
OWN - NLM
STAT- MEDLINE
DCOM- 19761203
LR  - 20091027
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 6
IP  - 1
DP  - 1976 Jan
TI  - Requirement for accessory cells in the antibody response to T cell-independent 
      antigens in vitro.
PG  - 63-8
AB  - The antibody responses of mouse spleen cells in vitro to three thymus-independent 
      (TI) antigens namely, polymeric flagellin (POL) of Salmonella adelaide, 
      DNP-Ficoll and "soluble" sheep erythrocyte (SRBC) antigen, were found to be 
      dependent on adherent accessory cells (A cells) but to a lesser degree than the 
      response to intact SRBC. Evidence for this comes from selective depletion of A 
      cells from spleen cells and reconstitution with A cell-rich populations. Thus, 
      depletion of A cells by adherence on glass resulted in abolition of the response 
      to SRBC leaving the response to POL intact. More thorough removal of A cells by 
      treatment with carbonyl iron powder was required for appreciable reduction of the 
      responses to POL, DNP-Ficoll and "soluble" SRBC. This reduction in responsiveness 
      was not due to poor cell survival after A cell depletion or to the loss of 
      immunocompetent cells since 1) the recoveries of viable cells in all cultures 
      were similar; 2) the contents of theta- and Ig-bearing cells and tritium-labeled 
      POL-binding cells were unaltered after carbonyl iron treatment, and 3) 
      responsiveness was fully restored by the addition of irradiated and 
      anti-theta-treated A cells from the peritoneal cavity or the spleen. Hence, the 
      hitherto A cell independence of TI antigens on which some theories of B cell 
      activation are based is a result of inadequate depletion procedures, and the 
      minimal model for B cell activation must take into account two cell types: B 
      cells and A cells.
FAU - Lee, K C
AU  - Lee KC
FAU - Shiozawa, C
AU  - Shiozawa C
FAU - Shaw, A
AU  - Shaw A
FAU - Diener, E
AU  - Diener E
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 12777-81-0 (Flagellin)
RN  - 25702-74-3 (Ficoll)
RN  - 60-24-2 (Mercaptoethanol)
SB  - IM
MH  - Animals
MH  - *Antibody Formation/drug effects
MH  - Ascitic Fluid/immunology
MH  - B-Lymphocytes/immunology
MH  - Cell Adhesion
MH  - Cell Separation
MH  - Erythrocytes/immunology
MH  - Female
MH  - Ficoll/immunology
MH  - Flagellin/immunology
MH  - Macrophages/*immunology
MH  - Male
MH  - Mercaptoethanol/pharmacology
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Spleen/immunology
MH  - T-Lymphocytes/*immunology
EDAT- 1976/01/01 00:00
MHDA- 1976/01/01 00:01
CRDT- 1976/01/01 00:00
PHST- 1976/01/01 00:00 [pubmed]
PHST- 1976/01/01 00:01 [medline]
PHST- 1976/01/01 00:00 [entrez]
AID - 10.1002/eji.1830060114 [doi]
PST - ppublish
SO  - Eur J Immunol. 1976 Jan;6(1):63-8. doi: 10.1002/eji.1830060114.