PMID- 10859591
OWN - NLM
STAT- MEDLINE
DCOM- 20001002
LR  - 20151119
IS  - 0143-4160 (Print)
IS  - 0143-4160 (Linking)
VI  - 27
IP  - 5
DP  - 2000 May
TI  - Purinergic responses in HT29 colonic epithelial cells are mediated by G protein 
      alpha -subunits.
PG  - 247-55
AB  - Using Fura-2 to measure changes in intracellular calcium ([Ca(2+)](i)), we show 
      that P(2U)receptors in HT29 cells trigger an increase in [Ca(2+)](i)by pertussis 
      toxin-insensitive G proteins. We then use replication-deficient adenoviruses 
      expressing wild-type and dominant negative mutants of G(alpha q)and G(alpha i2), 
      antisense directed against G(alpha q)or the C-terminal fragment of 
      beta-adrenergic receptor kinase (beta ARK-CT) to identify these G proteins. We 
      find the [Ca(2+)](i)response to UTP is not affected by increased expression of 
      the wild-type G(alpha q), wild-type G(alpha i2)or beta ARK-CT, while it is 
      blocked by over-expression of dominant negative G(alpha q). The timecourse of the 
      UTP response is, however, altered by wild-type G(alpha q)and is only weakly 
      inhibited by antisense G(alpha q). This suggests that the P(2U)response is 
      mediated, at least partially, by a G protein distinct from G(alpha q). In 
      contrast, the M(3)muscarinic response is inhibited by over-expression of 
      antisense against G(alpha q), or over-expression of beta ARK-CT, a finding in 
      agreement with our previous observation that the muscarinic response in HT29 
      cells is mediated by the beta gamma-subunits of G(q). We also find that P(2U)and 
      M(3)receptors do not control identical Ca(2+)stores, suggesting that differential 
      activation of G proteins can lead to Ca(2+)release from distinct stores.
CI  - Copyright 2000 Harcourt Publishers Ltd.
FAU - Cummins, M M
AU  - Cummins MM
AD  - Department of Physiology, University of Sydney, NSW, 2006, Australia.
FAU - O'Mullane, L M
AU  - O'Mullane LM
FAU - Barden, J A
AU  - Barden JA
FAU - Cook, D I
AU  - Cook DI
FAU - Poronnik, P
AU  - Poronnik P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Cell Calcium
JT  - Cell calcium
JID - 8006226
RN  - 0 (Cholinergic Agonists)
RN  - 0 (DNA, Antisense)
RN  - 0 (P2RY2 protein, human)
RN  - 0 (Receptor, Muscarinic M3)
RN  - 0 (Receptors, Muscarinic)
RN  - 0 (Receptors, Purinergic P2)
RN  - 0 (Receptors, Purinergic P2Y2)
RN  - 8Y164V895Y (Carbachol)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - EC 2.7.11.15 (beta-Adrenergic Receptor Kinases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - SY7Q814VUP (Calcium)
RN  - UT0S826Z60 (Uridine Triphosphate)
SB  - IM
MH  - Adenoviridae
MH  - Calcium/*metabolism
MH  - Carbachol/pharmacology
MH  - Cholinergic Agonists/pharmacology
MH  - Cyclic AMP-Dependent Protein Kinases/drug effects/metabolism
MH  - DNA, Antisense/pharmacology
MH  - GTP-Binding Proteins/drug effects/*metabolism
MH  - HT29 Cells/drug effects
MH  - Humans
MH  - Receptor, Muscarinic M3
MH  - Receptors, Muscarinic/drug effects/*metabolism
MH  - Receptors, Purinergic P2/drug effects/*metabolism
MH  - Receptors, Purinergic P2Y2
MH  - Uridine Triphosphate/pharmacology
MH  - beta-Adrenergic Receptor Kinases
EDAT- 2000/06/22 10:00
MHDA- 2000/10/07 11:01
CRDT- 2000/06/22 10:00
PHST- 2000/06/22 10:00 [pubmed]
PHST- 2000/10/07 11:01 [medline]
PHST- 2000/06/22 10:00 [entrez]
AID - S0143-4160(00)90120-9 [pii]
AID - 10.1054/ceca.2000.0120 [doi]
PST - ppublish
SO  - Cell Calcium. 2000 May;27(5):247-55. doi: 10.1054/ceca.2000.0120.