PMID- 10859938
OWN - NLM
STAT- MEDLINE
DCOM- 20000808
LR  - 20051117
IS  - 1343-4292 (Print)
IS  - 1343-4292 (Linking)
IP  - 117
DP  - 1999
TI  - Studies on karyotype evolution in higher primates in relation to human chromosome 
      14 and 9 by comparative mapping of immunoglobulin C epsilon genes with 
      fluorescence in situ hybridization.
PG  - 77-90
AB  - Karyotypic homologies in relation to human chromosome 14 and 9 were studied 
      through comparative mapping of the immunoglobulin C epsilon genes in higher 
      primates by fluorescence in situ hybridization (FISH) technique. The C epsilon 
      genes will be suitable probes for the analysis of evolutionary rearrangements due 
      to that the multiple recombinational events such as gene duplications and 
      deletions have occurred repeatedly in the immunoglobulin CH gene family (IGH@) 
      during the course of primate evolution. IGH@ locating on the terminal region of 
      human chromosome 14 (HSA14), at band HSA14q32.33, has generated multiple 
      pseudogenes and among subclasses of IGH@ the C epsilon genes have shown most 
      dynamic changes with generating both truncated type (C epsilon 2) and processed 
      type (C epsilon 3) pseudogenes. In this study, chromosomal homologies and 
      rearrangements on HSA14 (C epsilon 1) and HSA9 (C epsilon 3) in relation to the 
      evolutionary genesis of their primate homologous chromosomes in speciation were 
      investigated by comparative mapping with FISH and chromosome painting (ZOO-FISH) 
      techniques. Comparative mapping of the C epsilon 1 gene at HSA14q32.33 was 
      carried out in seven species of nonhuman primates: common chimpanzee (PTR), pygmy 
      chimpanzee (PPA), gorilla (GGO), orangutan (PPY), white-handed gibbon (HLA), 
      agile gibbon (HAG), and Japanese macaque (MFU). The C epsilon 1 gene was assigned 
      to the telomeric region of HSA14 homologues in each species, namely, PTR15q32, 
      PPA15q32, GGO18q16, PPY15q32, HLA17qter, HAG17qter, and MFU7q29, respectively. 
      These results suggested that HSA14 has high degree of syntenic organization with 
      its primate homologues confirmed by ZOO-FISH. Concerning HSA9, comparative 
      mapping of the C epsilon 3 gene at HSA9p24.2-->p24.1 was performed. The mapped 
      positions indicated the HSA9 homologous regions detected by ZOO-FISH in each 
      species, namely, PTR11q34, PPA11q34, GGO13q22, PPY13q16, HLA8qter, HAG8qter, and 
      MFU14q22, respectively, suggesting that several dynamic chromosomal 
      rearrangements including at least twice pericentric inversions have occurred 
      during the course of hominoid evolution. The comparison of syntenic groups and 
      painting results has provided a hypothesis of the evolutionary genesis of HSA9 
      and its homologues with defined breakpoints on the present chromosomes. Likewise, 
      studies on karyotype evolution will be promoted by combining comparative mapping 
      with ZOO-FISH that can more clearly define the chromosomal rearrangements among 
      species.
FAU - Tanabe, H
AU  - Tanabe H
AD  - tanabe@nihs.go.jp
LA  - eng
PT  - Journal Article
PT  - Review
PL  - Japan
TA  - Kokuritsu Iyakuhin Shokuhin Eisei Kenkyusho Hokoku
JT  - Kokuritsu Iyakuhin Shokuhin Eisei Kenkyujo hokoku = Bulletin of National 
      Institute of Health Sciences
JID - 9807834
RN  - 0 (Immunoglobulin epsilon-Chains)
SB  - IM
MH  - Animals
MH  - *Chromosome Mapping
MH  - Chromosomes, Human, Pair 14/*genetics
MH  - Chromosomes, Human, Pair 9/*genetics
MH  - Evolution, Molecular
MH  - Humans
MH  - Immunoglobulin epsilon-Chains/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Primates/*genetics
RF  - 116
EDAT- 2000/06/22 10:00
MHDA- 2000/08/12 11:00
CRDT- 2000/06/22 10:00
PHST- 2000/06/22 10:00 [pubmed]
PHST- 2000/08/12 11:00 [medline]
PHST- 2000/06/22 10:00 [entrez]
PST - ppublish
SO  - Kokuritsu Iyakuhin Shokuhin Eisei Kenkyusho Hokoku. 1999;(117):77-90.