PMID- 10860885
OWN - NLM
STAT- MEDLINE
DCOM- 20000802
LR  - 20061115
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 271
IP  - 2
DP  - 2000 Jun 5
TI  - Structure of transcripts and proteins encoded by U79-80 of human herpesvirus 6 
      and its subcellular localization in infected cells.
PG  - 307-20
AB  - We analyzed the U79-80 gene of human herpesvirus 6 (HHV-6), which is predicted to 
      be a positional homolog of the UL112-113 gene of human cytomegalovirus (HCMV). 
      The U79-80 gene encoded a family of nuclear proteins of 36, 41, 44, and 59 kDa. 
      These proteins had common amino termini and were generated by complex alternative 
      splicing. Transcripts from U79-80 appeared as early as 3 h postinfection and 
      could be detected in the presence of phosphonoformate. U79-80 proteins were seen 
      as early as 8 h postinfection and could be detected in the presence of 
      phosphonoformate but not in the presence of cycloheximide combined with 
      actinomycin D treatment. The U79-80 proteins were localized to the nucleus of 
      infected cells, where they were detected as a speckled or punctuate pattern. 
      Moreover, the U79-80 proteins colocalized with the components of the viral DNA 
      replication machinery and appeared to distribute adjacent to or touching nuclear 
      domain 10, where viral DNA replication occurs. From the sequence analysis of 
      genomic DNA, the predicted amino acid similarity between U79-80 and UL112-113 was 
      lower than between other genes, but the characteristics of the transcripts and 
      proteins encoded by U79-80 were similar to those of UL112-113. These results 
      suggest that the U79-80 proteins have a role in viral DNA replication and are 
      functional homologues of the UL112-113 proteins.
CI  - Copyright 2000 Academic Press.
FAU - Taniguchi, T
AU  - Taniguchi T
AD  - Department of Microbiology, Osaka University Medical School, Japan.
FAU - Shimamoto, T
AU  - Shimamoto T
FAU - Isegawa, Y
AU  - Isegawa Y
FAU - Kondo, K
AU  - Kondo K
FAU - Yamanishi, K
AU  - Yamanishi K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Line, Transformed
MH  - Cells, Cultured
MH  - Gene Expression
MH  - *Genes, Viral
MH  - Herpesvirus 6, Human/*genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Protein Processing, Post-Translational
MH  - RNA, Messenger
MH  - RNA, Viral
MH  - Rabbits
MH  - Subcellular Fractions
MH  - Transfection
MH  - Viral Proteins/*genetics/metabolism
EDAT- 2000/06/22 10:00
MHDA- 2000/08/06 11:00
CRDT- 2000/06/22 10:00
PHST- 2000/06/22 10:00 [pubmed]
PHST- 2000/08/06 11:00 [medline]
PHST- 2000/06/22 10:00 [entrez]
AID - S0042-6822(00)90326-X [pii]
AID - 10.1006/viro.2000.0326 [doi]
PST - ppublish
SO  - Virology. 2000 Jun 5;271(2):307-20. doi: 10.1006/viro.2000.0326.