PMID- 10861097
OWN - NLM
STAT- MEDLINE
DCOM- 20000731
LR  - 20190515
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 165
IP  - 1
DP  - 2000 Jul 1
TI  - Flt3-ligand and granulocyte colony-stimulating factor mobilize distinct human 
      dendritic cell subsets in vivo.
PG  - 566-72
AB  - Dendritic cells (DCs) have a unique ability to stimulate naive T cells. Recent 
      evidence suggests that distinct DC subsets direct different classes of immune 
      responses in vitro and in vivo. In humans, the monocyte-derived CD11c+ DCs induce 
      T cells to produce Th1 cytokines in vitro, whereas the CD11c- plasmacytoid T 
      cell-derived DCs elicit the production of Th2 cytokines. In this paper we report 
      that administration of either Flt3-ligand (FL) or G-CSF to healthy human 
      volunteers dramatically increases distinct DC subsets, or DC precursors, in the 
      blood. FL increases both the CD11c+ DC subset (48-fold) and the CD11c- IL-3R+ DC 
      precursors (13-fold). In contrast, G-CSF only increases the CD11c- precursors 
      (>7-fold). Freshly sorted CD11c+ but not CD11c- cells stimulate CD4+ T cells in 
      an allogeneic MLR, whereas only the CD11c- cells can be induced to secrete high 
      levels of IFN-alpha, in response to influenza virus. CD11c+ and CD11c- cells can 
      mature in vitro with GM-CSF + TNF-alpha or with IL-3 + CD40 ligand, respectively. 
      These two subsets up-regulate MHC class II costimulatory molecules as well as the 
      DC maturation marker DC-lysosome-associated membrane protein, and they stimulate 
      naive, allogeneic CD4+ T cells efficiently. These two DC subsets elicit distinct 
      cytokine profiles in CD4+ T cells, with the CD11c- subset inducing higher levels 
      of the Th2 cytokine IL-10. The differential mobilization of distinct DC subsets 
      or DC precursors by in vivo administration of FL and G-CSF offers a novel 
      strategy to manipulate immune responses in humans.
FAU - Pulendran, B
AU  - Pulendran B
AD  - Baylor Institute for Immunology Research, Dallas, TX 75204, USA.
FAU - Banchereau, J
AU  - Banchereau J
FAU - Burkeholder, S
AU  - Burkeholder S
FAU - Kraus, E
AU  - Kraus E
FAU - Guinet, E
AU  - Guinet E
FAU - Chalouni, C
AU  - Chalouni C
FAU - Caron, D
AU  - Caron D
FAU - Maliszewski, C
AU  - Maliszewski C
FAU - Davoust, J
AU  - Davoust J
FAU - Fay, J
AU  - Fay J
FAU - Palucka, K
AU  - Palucka K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Cytokines)
RN  - 0 (Integrin alphaXbeta2)
RN  - 0 (Interferon-alpha)
RN  - 0 (Ligands)
RN  - 0 (Membrane Proteins)
RN  - 0 (flt3 ligand protein)
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
SB  - IM
MH  - Adjuvants, Immunologic/*administration & dosage/*physiology
MH  - Adult
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - Cell Differentiation/immunology
MH  - Cytokines/biosynthesis
MH  - Dendritic Cells/cytology/*immunology/metabolism
MH  - Fetal Blood/cytology
MH  - Granulocyte Colony-Stimulating Factor/*administration & dosage/*physiology
MH  - Hematopoietic Stem Cells/immunology/metabolism
MH  - Humans
MH  - Injections, Subcutaneous
MH  - Integrin alphaXbeta2/biosynthesis
MH  - Interferon-alpha/biosynthesis
MH  - Ligands
MH  - Lymphocyte Activation
MH  - Lymphocyte Culture Test, Mixed
MH  - *Membrane Proteins/*administration & dosage/*physiology
MH  - T-Lymphocyte Subsets/immunology/metabolism
EDAT- 2000/06/22 10:00
MHDA- 2000/08/06 11:00
CRDT- 2000/06/22 10:00
PHST- 2000/06/22 10:00 [pubmed]
PHST- 2000/08/06 11:00 [medline]
PHST- 2000/06/22 10:00 [entrez]
AID - ji_v165n1p566 [pii]
AID - 10.4049/jimmunol.165.1.566 [doi]
PST - ppublish
SO  - J Immunol. 2000 Jul 1;165(1):566-72. doi: 10.4049/jimmunol.165.1.566.