PMID- 10861572
OWN - NLM
STAT- MEDLINE
DCOM- 20000713
LR  - 20041117
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 191
IP  - 2
DP  - 2000 Jun
TI  - c-erbB2 oncoprotein expression, gene amplification, and chromosome 17 aneusomy in 
      apocrine adenosis of the breast.
PG  - 138-42
AB  - Amplification of the c-erbB2 oncogene and numerical aberrations of chromosome 17 
      occur in human breast carcinomas. Apocrine adenosis (AA) of the breast has been 
      shown occasionally to have c-erbB2 overexpression and a possible premalignant 
      potential, but little is known about cellular level genetic alterations in AA of 
      the breast. Fluorescence in situ hybridization (FISH) is a new approach to detect 
      these. In this study, a series of AA was studied by immunohistochemistry for 
      c-erbB2 protein expression and by FISH using dual colour DNA probes for the 
      c-erbB2 gene and the centromeric region of chromosome 17. Cell membrane 
      immunostaining was seen in 10/18 (55.6%) AA cases, but unequivocal c-erbB2 gene 
      amplification or chromosome 17 aneusomy was not seen. The results of this study 
      suggest that c-erbB2 overexpression without amplification may occur early in 
      breast oncogenesis. Amplification and numerical chromosome aberrations may occur 
      later in the pathogenesis of apocrine-derived breast carcinomas.
CI  - Copyright 2000 John Wiley & Sons, Ltd.
FAU - Selim, A G
AU  - Selim AG
AD  - Department of Histopathology, St. Bartholomew's Hospital, St. Bartholomew's and 
      the Royal London School of Medicine and Dentistry, University of London, UK. 
      a.a.selim@mds.qmw.ac.uk
FAU - El-Ayat, G
AU  - El-Ayat G
FAU - Wells, C A
AU  - Wells CA
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
RN  - 0 (DNA Probes)
RN  - 0 (Oncogene Proteins v-erbB)
SB  - IM
MH  - Adult
MH  - *Aneuploidy
MH  - Cell Membrane/genetics
MH  - Centromere
MH  - Chromosomes, Human, Pair 17/*genetics
MH  - DNA Probes
MH  - Female
MH  - Fibrocystic Breast Disease/*genetics
MH  - *Gene Amplification
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - Oncogene Proteins v-erbB/*genetics
MH  - Precancerous Conditions/genetics
EDAT- 2000/06/22 10:00
MHDA- 2000/07/15 11:00
CRDT- 2000/06/22 10:00
PHST- 2000/06/22 10:00 [pubmed]
PHST- 2000/07/15 11:00 [medline]
PHST- 2000/06/22 10:00 [entrez]
AID - 10.1002/(SICI)1096-9896(200006)191:2<138::AID-PATH611>3.0.CO;2-J [pii]
AID - 10.1002/(SICI)1096-9896(200006)191:2<138::AID-PATH611>3.0.CO;2-J [doi]
PST - ppublish
SO  - J Pathol. 2000 Jun;191(2):138-42. doi: 
      10.1002/(SICI)1096-9896(200006)191:2<138::AID-PATH611>3.0.CO;2-J.