PMID- 10862039
OWN - NLM
STAT- MEDLINE
DCOM- 20000906
LR  - 20191025
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 28
IP  - 3
DP  - 2000 Jul
TI  - Chromosomal regions involved in the pathogenesis of osteosarcomas.
PG  - 329-36
AB  - The comparative genomic hybridization technique (CGH) was used to identify common 
      chromosomal imbalances in osteosarcomas (OS), which frequently display complex 
      karyotypic changes. We analyzed 13 high-grade primary tumors, 5 corresponding 
      cell lines, 2 primary tumors grade 2, and 1 recurrent tumor from a total of 16 
      patients. Some of the CGH results have been verified by fluorescence in situ 
      hybridization (FISH) studies. Gains of chromosomal material were more frequent 
      than losses. Most common gains were observed at 8q (11 cases), 4q (9 cases), 7q 
      (8 cases), 5p (7 cases), and 1p (8 cases). The smallest regions of overlap have 
      been narrowed down to 8q23 (10 cases), 4q12-13 (8 cases), 5p13-14 (7 cases), 
      7q31-32 (7 cases), 8q21 (7 cases), and 4q28-31 (5 cases). These data demonstrate 
      that a number of chromosomal regions and even two distinct loci on 4q and 8q are 
      involved in the pathogenesis of OS, with gain of 4q12-13 chromosomal material 
      representing a newly identified locus. Seven of 16 cases displayed, besides gain 
      of 8q23 sequences, gain of MYC copies in CGH and FISH. Previous CGH reports 
      confined gain of 8q material to 8cen-q13, 8q21.3-8q22, and 8q23-qter, whereas our 
      data suggest that the loci 8q21 and 8q23-24 are affected in the development of 
      OS. In contrast to recent reports, copy number increases at 8q and 1q21 did not 
      have an unfavorable impact on prognosis in the present series. Genes Chromosomes 
      Cancer 28:329-336, 2000.
CI  - Copyright 2000 Wiley-Liss, Inc.
FAU - Stock, C
AU  - Stock C
AD  - CCRI, St. Anna Children's Hospital, Vienna, Austria.
FAU - Kager, L
AU  - Kager L
FAU - Fink, F M
AU  - Fink FM
FAU - Gadner, H
AU  - Gadner H
FAU - Ambros, P F
AU  - Ambros PF
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Bone Neoplasms/*etiology/*genetics
MH  - Child
MH  - Chromosome Aberrations/*etiology/*genetics
MH  - Chromosome Disorders
MH  - Chromosome Painting
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Nucleic Acid Hybridization
MH  - Osteosarcoma/*etiology/*genetics
EDAT- 2000/06/22 10:00
MHDA- 2000/09/09 11:01
CRDT- 2000/06/22 10:00
PHST- 2000/06/22 10:00 [pubmed]
PHST- 2000/09/09 11:01 [medline]
PHST- 2000/06/22 10:00 [entrez]
AID - 10.1002/1098-2264(200007)28:3<329::AID-GCC11>3.0.CO;2-F [pii]
AID - 10.1002/1098-2264(200007)28:3<329::aid-gcc11>3.0.co;2-f [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2000 Jul;28(3):329-36. doi: 
      10.1002/1098-2264(200007)28:3<329::aid-gcc11>3.0.co;2-f.