PMID- 10864017
OWN - NLM
STAT- MEDLINE
DCOM- 20001018
LR  - 20190719
IS  - 0918-6158 (Print)
IS  - 0918-6158 (Linking)
VI  - 23
IP  - 6
DP  - 2000 Jun
TI  - Inhibitory effects of green tea and grape juice on the phenol sulfotransferase 
      activity of mouse intestines and human colon carcinoma cell line, Caco-2.
PG  - 695-9
AB  - Tea and fruit juices are beverages consumed daily all over the world. The present 
      study reports the inhibitory effects of these beverages on the activity of 
      mammalian intestinal phenol sulfotransferases (P-STs). Green tea strongly 
      inhibited the E. coli-expressed mouse intestinal P-ST activity in vitro. 
      (-)-Epigallocatechin gallate (EGCG) was found to be the most potent inhibitor 
      among the catechins tested (IC50=0.93 microM). (-)EGCG also inhibited the P-ST 
      activity of the human colon carcinoma cell line, Caco-2. Kinetic analysis showed 
      that the inhibition was competitive. Among fruit juices examined (apple, grape, 
      grapefruit and orange), grape juice exhibited the most potent inhibitory action 
      on the P-ST activity of mouse intestines and human colon carcinoma cells. The 
      inhibitory activity of grape juice was located mainly in the skin and seeds. 
      Flavonols, such as quercetin and kaempferol, inhibited the P-ST activity at low 
      concentrations. These observations suggest the possible inhibition of P-ST 
      activity in human intestines by green tea or grape juice.
FAU - Tamura, H
AU  - Tamura H
AD  - Kyoritsu College of Pharmacy, Tokyo, Japan. tamura-hr@kyoritsu-ph.ac.jp
FAU - Matsui, M
AU  - Matsui M
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Tea)
RN  - EC 2.8.2.1 (Arylsulfotransferase)
SB  - IM
MH  - Animals
MH  - Arylsulfotransferase/*antagonists & inhibitors
MH  - Beverages/*analysis
MH  - Caco-2 Cells
MH  - Colonic Neoplasms/*enzymology/pathology
MH  - Enzyme Inhibitors/isolation & purification/pharmacology
MH  - Fruit
MH  - Humans
MH  - Intestines/*enzymology
MH  - Male
MH  - Mice
MH  - Tea/*chemistry
EDAT- 2000/06/23 11:00
MHDA- 2000/10/21 11:01
CRDT- 2000/06/23 11:00
PHST- 2000/06/23 11:00 [pubmed]
PHST- 2000/10/21 11:01 [medline]
PHST- 2000/06/23 11:00 [entrez]
AID - 10.1248/bpb.23.695 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2000 Jun;23(6):695-9. doi: 10.1248/bpb.23.695.